The Bioinformatics Workshop - University College London

Date:

  Tuesday 28 May 2013

Application opens: 

Wednesday 13 March 2013

Application deadline: 

Friday 26 April 2013

Contact: 

Johanna Langrish

Registration closed

Overview

This course is intended to give life scientists training in two major areas: analysis of next generation sequencing data (NGS) as well as interactions and network analysis.

The first two days are designed to familiarize participants with NGS data analysis and to provide hands-on computational experience in analysis of such data using common analytical approaches for ChIP-seq and RNA-Seq data handling.

The third day provides an introduction to the study of Protein-Protein Interaction (PPI) networks, their importance and the techniques that are used to build and analyze them. Data resources related to pathways will also be covered.

 What will I learn?  

 •      What are the fundamental principles in the analysis of RNA-Seq and ChIP-Seq data. Topics will include: data quality, alignment, data handling and visualization, region identification, transcriptome analysis and basis statistical analysis.

 •      To explore the ENCODE data, the Encyclopedia of DNA Elements, a project aimed at identifying all regions of transcription, transcription factor association, chromatin structure and histone modification in the human genome sequence.

 •      To understand what is to be found in a molecular interaction database, alternative data formats and the various tools designed to work with them.

 •      To learn how to explore reactions and pathways.

Programme

Time Topic  
Day 1 - 28 May - RNA-seq data analysis  -   DAY 1 - NOW FULLY BOOKED
09:00 - 09:10 Welcome and introduction  
09:10 - 10:15 Lecture: Transcriptome assembly and differential expression analysis Myrto Kostadima/Remco Loos
10:15 - 10:30 Tea/coffee break  
10:30 - 12:00 Practical: Reference guided transcriptome assembly Myrto Kostadima/Remco Loos
12:00 - 13:00 Lunch  
13:00 - 15:15 Practical: Differential expression analysis Myrto Kostadima/Remco Loos
15:15 - 15:30 Tea/coffee break  
15:30 - 17:00 Lecture/Practical: Downstream analysis Myrto Kostadima/Remco Loos
Day 2 - 29 May - ENCODE & ChIP-seq data analysis - DAY 2 - NOW FULLY BOOKED
09:00 - 10:00 Introduction to ENCODE data Ian Dunham/Steven Wilder
10:00 - 10:15 Tea/coffee break  
10:15 - 12:00 Working with ENCODE data Ian Dunham/Steven Wilder
12:00 - 13:00 Lunch  
13:00 - 14:00 Principals of NGS Data Analysis Myrto Kostadima/Remco Loos
14:00 - 15:30 Principals of NGS Data Analysis Myrto Kostadima/Remco Loos
15:30 - 15:45 Tea/coffee break  
15:45 - 17:00 Principals of NGS Data Analysis Myrto Kostadima/Remco Loos
Day 3 - 30 May - Interactions and Network analysis - DAY 3 - PLACES AVAILABLE
09:00 - 10:00 Introduction to protein-protein interactions (PPIs) and networks Sandra Orchard
10:00 - 10:15 Tea/coffee break  
10:15 - 12:30 A PPIs database: IntAct. Description and hands-on session. Includes a brief introduction to Cytscape Sandra Orchard
12:30 - 13:30 PPI data integration: PSICQUIC. Description and hands-on session with PSICQUICView Sandra Orchard
13:30 - 14:30 Lunch  
14:30 - 15:30 Reactome - description and hands-on Sandra Orchard
15:30 - 16:00 Tea/coffee break  
16:00 - 17:00 Reactome - description and hands-on (cont.) Sandra Orchard