RNA-Seq and ChIP-seq Data Analysis
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Date:
Wednesday 9 July 2014Application opens:
Tuesday 20 May 2014Application deadline:
Friday 06 June 2014Contact:
Rebecca GreenhaffRegistration closed
Overview
Is it right for me?
This course is aimed at advanced PhD students and post-doctoral researchers who are applying or planning to apply high throughput sequencing technologies and bioinformatics methods in their research. The aim of this course is to familiarise the participants with advanced data analysis methodologies and provide hands-on training on the latest analytical approaches.
What will I learn?
Lectures will give insight into how biological knowledge can be generated from RNA-seq and ChIP-seq experiments and illustrate different ways of analyzing such data Practicals will consist of computer exercises that will enable the participants to apply statistical methods to the analysis of RNA-seq and ChIP-seq data under the guidance of the lecturers and teaching assistants. Familiarity with the technology and biological use cases of high throughput sequencing is required, as is some experience with R/Bioconductor.
What will it cover?
The course covers data analysis of RNA-Seq and ChIP-Seq experiments.
Topics will include: alignment, data handling and visualisation, region identification, differential expression, data quality assessment and statistical analysis, using R/Bioconductor.
Programme
Time | Topic | Trainer |
---|---|---|
Day 1 - Wednesday 9 July - Introduction to sequencing and ChIP-seq analysis | ||
09:00 - 09:10 | Welcome and introduction | G. Rustici |
09:10 - 10:00 | Lecture: Next generation sequencing overview (+exercise) | R. Loos/M. Xenophontos |
10:00 - 10:15 | Tea/coffee break | |
10:15 - 10:45 | Lecture & Practical: NGS quality control | R. Loos/M. Xenophontos |
10:45 - 12:00 | Lecture & Practical: Sequence alignment | R. Loos/M. Xenophontos |
12:00 - 13:00 | Lunch | |
13:00 - 13:30 | Lecture: Introduction to ChIP-seq | R. Loos/M. Xenophontos |
13:30 - 15:00 | Practical: ChIP-seq - peak calling and annotation | R. Loos/M. Xenophontos |
15:00 - 15:15 | Tea/coffee break | |
15:15 - 17:00 | Practical: ChIP-seq - motif analysis | R. Loos/M. Xenophontos |
17:00 - 17:30 | DiXa project overview | V. Matser |
19:30 | Optional Course dinner at Wellcome Trust Centre | |
Day 2 - Thursday 10 July - RNA-seq data analysis | ||
09:00 - 10:00 | Lecture: Benchmarking of aligners for sequencing data | B. Sipos |
10:00 - 10:15 | Tea/coffee break | |
10:15 - 11:30 | Lecture: Introduction to RNA-seq | M. Kostadima/N. Koelling |
11:30 - 12:30 | Practical: RNA-seq - Alignment and splice junction identification | M. Kostadima/N. Koelling |
12:30 - 13:30 | Lunch | |
13:30 - 14:15 | Practical: RNA-seq - Transcriptome assembly | M. Kostadima/N. Koelling |
14:15 - 15:15 | Practical: RNA-seq analysis - Differential expression analysis | M. Kostadima/N. Koelling |
15:15 - 15:30 | Tea/coffee break | |
15:30 - 16:00 | Practical: RNA-seq analysis - Differential expression analysis | M. Kostadima/N. Koelling |
16:00 - 17:00 | Lecture: On the empirical evaluation of RNA-seq gene profiling pipelines COURSE FEEDBACK | N. Fonseca |