Course at EMBL-EBI
Livestock genomics
This course will introduce participants to methods and approaches for analysing genomic data from common livestock species. It will guide participants through understanding the theory of next generation sequencing methods, and how these are applied in the field of livestock genomics. Over the course participants will learn about resequencing genomes, locating variant data, annotating a genome sequence using transcriptomics data and how to begin ascribing function to genes and genomic regions. The content will also explain how to make use of existing public data, submit and share your discoveries with the community and draw on relevant use cases in the field.
Who is this course for?
This course is aimed at PhD students and postdoctoral researchers needing to learn about methods and approaches for manipulating and analysing livestock genomic data. It will help those wanting to start basic identification of genetic variation, annotating function to genomic data, and using public data to interpret new findings.
Participants will require a basic knowledge of the Unix command line, the Ubuntu 18 operating system and the R statistical packages. We recommend these free tutorials:
- Basic introduction to the Unix environment: www.ee.surrey.ac.uk/Teaching/Unix
- Introduction and exercises for Linux: https://training.linuxfoundation.org/free-linux-training
- Basic R concept tutorials: www.r-tutor.com/r-introduction
Regardless of your current knowledge we encourage successful participants to use these, and other materials, to prepare for attending the course and future work in this area.
What will I learn?
Learning outcomes
During this course you will learn about:
- Basics of NGS technologies
- Read mapping, variant calling and GWAS
- Genebuild/Ensembl Annotation
- Ensembl Browser + data resources for genomics
- Relevant genomics use cases in key livestock species
Course content
During this course you will learn about:
- Basics of NGS technologies
- Read mapping, variant calling and GWAS
- Genebuild/Ensembl Annotation
- Ensembl Browser + data resources for genomics
- Relevant use cases
Trainers
Piraveen Gopalasingam
EMBL-EBI, UK Thibaut Hourlier
EMBL-EBI, UK Francisco Lobo
Federal University of Minas Gerais (UFMG), Brazil Cath Brooksbank
EMBL-EBI, UK Alan Archibald
Roslin Institute, Edinburgh University, UK Sam Holt
EMBL-EBI, UK Baron Koylass
EMBL-EBI, UK Astrid Gall
EMBL-EBI, UK Juan Pablo Nani
INTA, Argentina Kostas Billis
EMBL-EBI, UK Jane Loveland
EMBL-EBI, UK Silvie Fexova
EMBL-EBI, UK Wendy Acuna
Instituto Nacional de Innovación Agraria (INIA), Perú Manuel More
Universidad Nacional Agraria La Molina, Perú Rafael Penha Filho
Sao Paulo State University (UNESP), Brazil Diana Lopez
Universidad Nacional de Colombia, Colombia Natalia Pin Viso
INTA Castelar, Buenos Aires, Argentina
Programme
Day 1 – Monday 1 April 2019
10:00-10:30
Arrival, registration and refreshments
10:30 -11:00
Welcome and introduction to EMBL-EBI
Piv Gopalasingam
11:00-12:00
Livestock genomics in Latin America and CABANA’s role in capacity strengthening
Cath Brooksbank
12:00-13:00
Introductions, networking and expectations
Piv Gopalasingam
13:00-14:00
Lunch and posters
14:00-15:00
Introduction to genomics
CABANA Trainers
15:00-15:30
Break
15:30-17:00
NGS bioinformatics file formats practical
CABANA Trainers
17:00-18:00
Using NGS methods for livestock genomics
CABANA Trainers
18:15
Check in to accommodation
19:30
Evening meal
Hinxton Hall Restaurant
Day 2 – Tuesday 2 April 2019
08:45
Arrival
09:00-10:00
Functional annotation of animal genomes and the importance of reference genomes for livestock research
Paul Flicek
10:00-10:30
Break
10:30-12:00
Genomes and manual annotation
Jane Loveland
12:00-13:00
Lunch and posters
13:00-15:00
Ensembl genome annotation
Thibaut Hourlier & Kostas Billis
15:00-16:00
Introduction to Ensembl
Astrid Gall
16:00-16:30
Break
16:30-18:30
Genes and transcripts and comparative genomics
Astrid Gall
19:00
Evening meal
Hinxton Hall Restaurant
Day 3 – Wednesday 3 April 2019
08:30
Arrival, registration
09:00-10:00
Forward and reverse genetics approaches to infectious disease in farmed animals
Alan Archibald
10:00-10:30
Break
10:30-11:30
Genome resequencing
Francisco Lobo
11:30-13:00
Resequencing and variant calling practical
Francisco Lobo
13:00-14:00
Lunch and posters
14:00-16:00
Resequencing and variant calling practical
Francisco Lobo
16:00-16:30
Break
16:30-17:30
Resequencing and variant calling practical
Francisco Lobo
17:30-18:30
Variation in Ensembl
Astrid Gall
18:30
End of day
19:30
Dinner
Hinxton Hall Restaurant
Day 4 – Thursday 4 April 2019
08:45
Arrival
09:00-10:00
Interpretation of variant effects using VEP
Astrid Gall
10:00-11:00
Genome Wide Association Studies
Juan Pablo Nani
11:00-11:15
Break
11:15-12:45
GWAS practical
Juan Pablo Nani
12:45-13:15
GWAS data resources
Juan Pablo Nani
13:15-14:00
Lunch and posters
14:00-16:00
Data resources for variant data: EVA
Baron Koylass
16:00-16:30
Break
16:30-18:30
BioMart, Data upload
Astrid Gall
18:30
End of day
19:30
Evening meal
Hinxton Hall Restaurant
Day 5 - Friday 5 April 2019
08:30
Check out of accommodation
08:45
Arrival
09:00-10:00
Discovering nucelotide sequences with ENA
Sam Holt
10:00-10:30
Break
10:30-11:45
Exploring gene expression data with Expression Atlas
Silvie Fexova
11:45-12:15
Course wrap-up and feedback
Piv Gopalasingam
12:15-13:00
Lunch
13:00
Shuttle to Cambridge Station
Please read our page on application advice before starting your application. In order to be considered for a place on this course, you must do the following:
- Complete the online application form
- Provide a Microsoft Word (.docx) document containing three short paragraphs with a biography, work history and description of your current research interests; each paragraph should be no more than 100 words
- Provide a letter of support from a supervisor or a senior co-worker explaining why you should be selected for this course
Incomplete applications will not be considered.
CABANA Travel Fellowships & Course Fee Bursary
The CABANA project has made available funds to provide 10 successful applicants from Latin American countries with a bursary to cover 100% of the course fee and up to £1125 towards travel costs. If you fulfill the selection criteria and wish to apply for a CABANA Travel Fellowship or Course Fee Bursary please complete this form in addition to your application.
Participants have the opportunity to display an A2 (420x594mm - portrait) poster for the duration of the course. We will ask you to submit your poster before the course for printing on site. It is not possible to display posters of a different size or orientation.
Accommodation will be provided in the Wellcome Genome Campus Conference Centre Monday-Friday inclusive. Please contact the Conference Centre directly if you wish to arrange to stay additional nights. The course fee includes breakfast and evening meals at Hinxton Hall, as well as breaks and lunches outside the EMBL-EBI training rooms.
EMBL-EBI, UK
EMBL-EBI, UK
Federal University of Minas Gerais (UFMG), Brazil
EMBL-EBI, UK
Roslin Institute, Edinburgh University, UK
EMBL-EBI, UK
EMBL-EBI, UK
EMBL-EBI, UK
INTA, Argentina
EMBL-EBI, UK
EMBL-EBI, UK
EMBL-EBI, UK
Instituto Nacional de Innovación Agraria (INIA), Perú
Universidad Nacional Agraria La Molina, Perú
Sao Paulo State University (UNESP), Brazil
Universidad Nacional de Colombia, Colombia
INTA Castelar, Buenos Aires, Argentina
Programme
Day 1 – Monday 1 April 2019 |
||
---|---|---|
10:00-10:30 | Arrival, registration and refreshments | |
10:30 -11:00 | Welcome and introduction to EMBL-EBI | Piv Gopalasingam |
11:00-12:00 | Livestock genomics in Latin America and CABANA’s role in capacity strengthening | Cath Brooksbank |
12:00-13:00 | Introductions, networking and expectations | Piv Gopalasingam |
13:00-14:00 | Lunch and posters | |
14:00-15:00 | Introduction to genomics | CABANA Trainers |
15:00-15:30 | Break | |
15:30-17:00 | NGS bioinformatics file formats practical | CABANA Trainers |
17:00-18:00 | Using NGS methods for livestock genomics | CABANA Trainers |
18:15 | Check in to accommodation | |
19:30 | Evening meal | Hinxton Hall Restaurant |
Day 2 – Tuesday 2 April 2019 |
||
---|---|---|
08:45 | Arrival | |
09:00-10:00 | Functional annotation of animal genomes and the importance of reference genomes for livestock research | Paul Flicek |
10:00-10:30 | Break | |
10:30-12:00 | Genomes and manual annotation | Jane Loveland |
12:00-13:00 | Lunch and posters | |
13:00-15:00 | Ensembl genome annotation | Thibaut Hourlier & Kostas Billis |
15:00-16:00 | Introduction to Ensembl | Astrid Gall |
16:00-16:30 | Break | |
16:30-18:30 | Genes and transcripts and comparative genomics | Astrid Gall |
19:00 | Evening meal | Hinxton Hall Restaurant |
Day 3 – Wednesday 3 April 2019 |
||
---|---|---|
08:30 | Arrival, registration | |
09:00-10:00 | Forward and reverse genetics approaches to infectious disease in farmed animals | Alan Archibald |
10:00-10:30 | Break | |
10:30-11:30 | Genome resequencing | Francisco Lobo |
11:30-13:00 | Resequencing and variant calling practical | Francisco Lobo |
13:00-14:00 | Lunch and posters | |
14:00-16:00 | Resequencing and variant calling practical | Francisco Lobo |
16:00-16:30 | Break | |
16:30-17:30 | Resequencing and variant calling practical | Francisco Lobo |
17:30-18:30 | Variation in Ensembl | Astrid Gall |
18:30 | End of day | |
19:30 | Dinner | Hinxton Hall Restaurant |
Day 4 – Thursday 4 April 2019 |
||
---|---|---|
08:45 | Arrival | |
09:00-10:00 | Interpretation of variant effects using VEP | Astrid Gall |
10:00-11:00 | Genome Wide Association Studies | Juan Pablo Nani |
11:00-11:15 | Break | |
11:15-12:45 | GWAS practical | Juan Pablo Nani |
12:45-13:15 | GWAS data resources | Juan Pablo Nani |
13:15-14:00 | Lunch and posters | |
14:00-16:00 | Data resources for variant data: EVA | Baron Koylass |
16:00-16:30 | Break | |
16:30-18:30 | BioMart, Data upload | Astrid Gall |
18:30 | End of day | |
19:30 | Evening meal | Hinxton Hall Restaurant |
Day 5 - Friday 5 April 2019 |
||
---|---|---|
08:30 | Check out of accommodation | |
08:45 | Arrival | |
09:00-10:00 | Discovering nucelotide sequences with ENA | Sam Holt |
10:00-10:30 | Break | |
10:30-11:45 | Exploring gene expression data with Expression Atlas | Silvie Fexova |
11:45-12:15 | Course wrap-up and feedback | Piv Gopalasingam |
12:15-13:00 | Lunch | |
13:00 | Shuttle to Cambridge Station |
Please read our page on application advice before starting your application. In order to be considered for a place on this course, you must do the following:
- Complete the online application form
- Provide a Microsoft Word (.docx) document containing three short paragraphs with a biography, work history and description of your current research interests; each paragraph should be no more than 100 words
- Provide a letter of support from a supervisor or a senior co-worker explaining why you should be selected for this course
Incomplete applications will not be considered.
CABANA Travel Fellowships & Course Fee Bursary
The CABANA project has made available funds to provide 10 successful applicants from Latin American countries with a bursary to cover 100% of the course fee and up to £1125 towards travel costs. If you fulfill the selection criteria and wish to apply for a CABANA Travel Fellowship or Course Fee Bursary please complete this form in addition to your application.
Participants have the opportunity to display an A2 (420x594mm - portrait) poster for the duration of the course. We will ask you to submit your poster before the course for printing on site. It is not possible to display posters of a different size or orientation.
Accommodation will be provided in the Wellcome Genome Campus Conference Centre Monday-Friday inclusive. Please contact the Conference Centre directly if you wish to arrange to stay additional nights. The course fee includes breakfast and evening meals at Hinxton Hall, as well as breaks and lunches outside the EMBL-EBI training rooms.