Virtual course
CABANA workshop: Applied bioinformatics, molecular epidemiology, and the SARS-CoV-2 pandemic
This workshop will provide training on the use of bioinformatic tools for the design and analysis of research and diagnostic molecular assays. Trainees will receive training on viral genome assembly, comparative viral genomics and downstream applications of this data in research and applied contexts. Trainers will focus practical sessions on the analysis of Illumina SARS-CoV-2 sequences.
This online workshop will be delivered on Zoom and comprise a blend of live lectures and hands-on practicals delivered by trainers across Latin America. Certificates will be provided to mark successful completion of this workshop. Sessions on Friday 4th December will be livestreamed on YouTube and made freely available on the platform thereafter. There will be an opportunity to deliver a short presentation about your work to other trainees.
Who is this course for?
This course is suitable for postgraduate (MSc-level and above) scientists within Latin America (excluding Chile and Uruguay) who are working with or generating genomic datasets related to SARS-CoV-2. Ideally, trainees will be working in the field of virology, and performing sequencing and/or analysing viral sequences, especially those within the coronaviridae family. We welcome applications from universities, research centres, hospitals, public health, and private laboratories.
Scientists from underrepresented ethnic and gender groups are especially encouraged to apply for this workshop.
Please note this course will be taught in English, however the trainers are fluent in either Spanish or Portuguese and can offer language support where feasible. Attendance priority will be given to those who have not attended a CABANA event yet.
Prerequisites
Undergraduate-level knowledge of biology would be advantageous. You will also require access to a computer for the duration of the workshop. While most practicals are browser-based, a few will require a UNIX environment. Trainees will therefore require a basic knowledge of the Unix command line.
We recommend these free tutorials:
- Basic introduction to the Unix environment: www.ee.surrey.ac.uk/Teaching/Unix
- Introduction and exercises for Linux: https://training.linuxfoundation.org/free-linux-training
Training will be delivered via Zoom; trainees need to ensure they have working audio and microphones.
What will I learn?
Learning outcomes
After this course you should be able to:
- Know the main public repositories for virus sequences
- Analyse genomic sequences of viruses
- Design PCR primers following international recommendations
- Apply international standards for the reporting of molecular epidemiology studies
Course content
During this course you will learn about:
- Viral genome assembly
- Comparative analysis of viral genomes
- Primer design for qPCR
- Point of care molecular testing
- Standards for reporting of molecular epidemiology studies
Trainers
Marco Cristancho
Universidad de Los Andes, Colombia Diego Forero
Fundación Universitaria del Área Andina, Colombia Jan Kreuze
International Potato Center (CIP), Lima, Peru Segundo Fuentes
International Potato Center (CIP), Lima, Peru Gisele Nunes Lopes
Instituto Tecnológico Vale (ITV), Brazil Dario Fernández Do Porto
Instituto de Cálculo (IC), Universidad de Buenos Aires, Argentina Gilderlanio Santana de Araujo
Universidade Federal do Pará (UFPA), Brazil Priscila Born
Fundação Oswaldo Cruz (Fiocruz) Rio de Janeiro, Brazil Valentina Gallo
University of Groningen, Netherlands Agustin Pardo
FCEN, Universidad de Buenos Aires, Argentina Claudio Schuster
IQUIBICEN, Universidad de Buenos Aires, Argentina
Programme
Day 1 – Tuesday 1 December 2020
08:45-09:00
Welcome and introductions
Marco Cristancho & Diego Forero
09:00-09:45
Sequencing technologies (Theoretical)
Dario Fernandez
09:45-10:30
Viral genomes databases – GISAID and other repositories
Priscila Born
10:30-10:45
Break
10:45-11:30
DNA sequence Quality Control (Theoretical - Practical)
Gisele Nunes & Gilderlanio Araujo
11:30-12:00
Trainee presentations
Trainees
12:00
End of day 1
Day 2 – Wednesday 2 December 2020
09:00-11:00
Assembly of viral genomes (Theoretical - Practical)
Jan Kreuze & Segundo Fuentes
11:00-11:15
Break
11:15-12:00
Trainee presentations
Trainees
12:00
End of day 2
Day 3 – Thursday 3 December 2020
09:00-10:00
Comparative analysis of viral genomes (Theoretical - Practical)
Dario Fernandez
10:00-11:00
Phylogenomics of viral genomes (Theoretical - Practical)
Dario Fernandez
11:00-11:15
Break
11:15-11:45
Trainee presentations
Trainees
11:45
End of day 3
Day 4 – Friday 4 December 2020
08:45-09:30
Primer design for qPCR (Theoretical)
Segundo Fuentes & Ana Perez
09:30-10:15
Point of care molecular testing (Point of care isothermal amplification protocols) - LAMP (Theoretical)
Segundo Fuentes - Ana Perez
10:15-10:30
Break
10:30-11:15
Standards for reporting of molecular epidemiology studies - STROBE-ME (Theoretical)
Valentina Gallo
11:15-11:45
Trainee presentations
Trainees
11:45-12:00
End of workshop: feedback and certificates
Marco Cristancho & Diego Forero
In order to be considered for a place in this workshop applicants must complete the online application form.
Incomplete applications will NOT be considered.
If you have any queries about the application process please email Guilherme Oliveira and Piv Gopalasingam.
Please note there is NO registration fee and you will not be asked to pay for this workshop.
There will be a maximum of 30 trainees on this workshop and registration is dependent on selection after successful completion of the application process.
Applications will close on Monday 16th November 2020 at 18:30 (COT).
Notification emails will be sent approximately 1 week after the application deadline.
Universidad de Los Andes, Colombia
Fundación Universitaria del Área Andina, Colombia
International Potato Center (CIP), Lima, Peru
International Potato Center (CIP), Lima, Peru
Instituto Tecnológico Vale (ITV), Brazil
Instituto de Cálculo (IC), Universidad de Buenos Aires, Argentina
Universidade Federal do Pará (UFPA), Brazil
Fundação Oswaldo Cruz (Fiocruz) Rio de Janeiro, Brazil
University of Groningen, Netherlands
FCEN, Universidad de Buenos Aires, Argentina
IQUIBICEN, Universidad de Buenos Aires, Argentina
Programme
Day 1 – Tuesday 1 December 2020 |
||
---|---|---|
08:45-09:00 | Welcome and introductions | Marco Cristancho & Diego Forero |
09:00-09:45 | Sequencing technologies (Theoretical) | Dario Fernandez |
09:45-10:30 | Viral genomes databases – GISAID and other repositories | Priscila Born |
10:30-10:45 | Break | |
10:45-11:30 | DNA sequence Quality Control (Theoretical - Practical) | Gisele Nunes & Gilderlanio Araujo |
11:30-12:00 | Trainee presentations | Trainees |
12:00 | End of day 1 |
Day 2 – Wednesday 2 December 2020 |
||
---|---|---|
09:00-11:00 | Assembly of viral genomes (Theoretical - Practical) | Jan Kreuze & Segundo Fuentes |
11:00-11:15 | Break | |
11:15-12:00 | Trainee presentations | Trainees |
12:00 | End of day 2 | |
Day 3 – Thursday 3 December 2020 |
||
09:00-10:00 | Comparative analysis of viral genomes (Theoretical - Practical) | Dario Fernandez |
10:00-11:00 | Phylogenomics of viral genomes (Theoretical - Practical) | Dario Fernandez |
11:00-11:15 | Break | |
11:15-11:45 | Trainee presentations | Trainees |
11:45 | End of day 3 | |
Day 4 – Friday 4 December 2020 |
||
08:45-09:30 | Primer design for qPCR (Theoretical) | Segundo Fuentes & Ana Perez |
09:30-10:15 | Point of care molecular testing (Point of care isothermal amplification protocols) - LAMP (Theoretical) | Segundo Fuentes - Ana Perez |
10:15-10:30 | Break | |
10:30-11:15 | Standards for reporting of molecular epidemiology studies - STROBE-ME (Theoretical) | Valentina Gallo |
11:15-11:45 | Trainee presentations | Trainees |
11:45-12:00 | End of workshop: feedback and certificates | Marco Cristancho & Diego Forero |
In order to be considered for a place in this workshop applicants must complete the online application form.
Incomplete applications will NOT be considered.
If you have any queries about the application process please email Guilherme Oliveira and Piv Gopalasingam.
Please note there is NO registration fee and you will not be asked to pay for this workshop.
There will be a maximum of 30 trainees on this workshop and registration is dependent on selection after successful completion of the application process.
Applications will close on Monday 16th November 2020 at 18:30 (COT).
Notification emails will be sent approximately 1 week after the application deadline.