This term is obsolete.

GO:0004822 JSON

isoleucine-tRNA ligase activity

Molecular Function

Definition (GO:0004822 GONUTS page)

Catalysis of the reaction: L-isoleucine + ATP + tRNA(Ile) = L-isoleucyl-tRNA(Ile) + AMP + diphosphate + 2 H+.

67,322 annotations

Synonyms

Synonyms are alternative words or phrases closely related in meaning to the term name, with indication of the relationship between the name and synonym given by the synonym scope.

Synonym Type
isoleucine-tRNA synthetase activity related
isoleucyl-tRNA synthetase activity exact
isoleucyl-transfer ribonucleate synthetase activity related
isoleucine-transfer RNA ligase activity related
isoleucyl-transfer RNA synthetase activity related
L-isoleucine:tRNAIle ligase (AMP-forming) related
isoleucine translase activity related

Ancestor Chart

Ancestor chart for GO:0004822
Chart options
Ancestor chart

Annotation Guidance

Usage of this term is subject to the following annotation guidelines:

Annotating to catalytic activities using the IPI evidence code Link
Describing enzyme functions that catalyse multi-stage reactions Link

Annotation Blacklist

This list aims to correct incorrect annotations to UniProtKB accessions inferred from electronic annotation (IEA) methods that are supplied by the UniProt-GOA project to the GO Consortium:

Category Entity Type Entity ID Taxon ID Entity Name Ancestor GO ID Reason Rule/Method ID
NOT-qualified manual protein A0A8I5Y5Z6 10116 A0A8I5Y5Z6_RAT GO:0016874 1 NOT-qualified manual annotation exists with evidence code ECO:0000266 from this reference: GO_REF:0000096
NOT-qualified manual protein A0A8I6A2T0 10116 A0A8I6A2T0_RAT GO:0016874 1 NOT-qualified manual annotation exists with evidence code ECO:0000266 from this reference: GO_REF:0000096
NOT-qualified manual protein A6I4N6 10116 A6I4N6_RAT GO:0016874 1 NOT-qualified manual annotation exists with evidence code ECO:0000266 from this reference: GO_REF:0000096
NOT-qualified manual protein F7EEB9 10116 F7EEB9_RAT GO:0016874 1 NOT-qualified manual annotation exists with evidence code ECO:0000266 from this reference: GO_REF:0000096
NOT-qualified manual protein P02521 9031 ARLY1_CHICK GO:0003824 1 NOT-qualified manual annotation exists with evidence code ECO:0000304 from this reference: PMID:11258884
NOT-qualified manual protein Q13426 9606 XRCC4_HUMAN GO:0016874 1 NOT-qualified manual annotation exists with evidence code ECO:0000314 from this reference: PMID:9242410
NOT-qualified manual protein Q7KGG1 7227 Q7KGG1_DROME GO:0003824 1 NOT-qualified manual annotation exists with evidence code ECO:0000320 from this reference: PMID:11904370
NOT-qualified manual protein Q7SIE0 9103 ARLY1_MELGA GO:0003824 1 NOT-qualified manual annotation exists with evidence code ECO:0000304 from this reference: PMID:11258884
NOT-qualified manual protein Q8MZ84 7227 Q8MZ84_DROME GO:0003824 1 NOT-qualified manual annotation exists with evidence code ECO:0000320 from this reference: PMID:11904370

Cross-references

Database ID Description
EC 6.1.1.5
InterPro IPR002301 Isoleucine-tRNA ligase
InterPro IPR023585 Isoleucine-tRNA ligase, type 1
InterPro IPR023586 Isoleucine-tRNA ligase, type 2
KEGG_REACTION R03656
MetaCyc ISOLEUCINE--TRNA-LIGASE-RXN
RHEA 11060
Reactome R-HSA-379893
Reactome R-HSA-380176

Cross-Ontology Relations

Relation Other Ontology ID Term
has_participant CHEBI CHEBI:33019 diphosphate(3-)
has_participant CHEBI CHEBI:456215 adenosine 5'-monophosphate(2-)
has_participant CHEBI CHEBI:78528 3'-(L-isoleucyl)adenylyl zwitterionic group
has_participant CHEBI CHEBI:58045 L-isoleucine zwitterion
has_participant CHEBI CHEBI:30616 ATP(4-)
has_participant CHEBI CHEBI:78442 AMP 3'-end(1-) residue

Co-occurring Terms

These tables show the number of times the term listed in the table has been co-annotated.

No co-occurrence statistics for GO:0004822 based on ALL annotations
The top 100 of 323 co-occurring terms
Co-occurring Term PR S% #Together #Compared
isoleucine-tRNA ligase activity
2,907.75 100.00 67,041 67,041
isoleucyl-tRNA aminoacylation
2,907.75 98.87 66,283 66,283
aminoacyl-tRNA editing activity
504.02 16.84 57,375 331,001
aminoacyl-tRNA metabolism involved in translational fidelity
449.00 15.05 57,375 371,564
tRNA aminoacylation for protein translation
172.23 5.30 22,588 381,347
aminoacyl-tRNA ligase activity
152.28 5.23 66,153 1,263,165
tRNA binding
123.22 4.20 54,747 1,291,960
mitochondrial translation
101.81 2.44 5,004 142,916
reproductive structure development
271.49 1.43 1,109 11,878
post-embryonic development
126.22 1.21 1,109 25,549
Totals 679537 127522390
No co-occurrence statistics for GO:0004822 based on MANUAL annotations
The top 32 of 32 co-occurring terms
Co-occurring Term PR S% #Together #Compared
isoleucine-tRNA ligase activity
5,065.88 100.00 255 255
isoleucyl-tRNA aminoacylation
5,065.88 99.61 254 254
mitochondrial translation
253.43 4.61 94 1,879
GTPase binding
176.97 1.68 8 229
aminoacyl-tRNA synthetase multienzyme complex
78.30 1.12 10 647
tRNA aminoacylation for protein translation
434.22 1.05 3 35
regulation of sprouting angiogenesis
72.37 0.31 1 70
glutamine-tRNA ligase activity
37.53 0.26 1 135
arginine-tRNA ligase activity
24.12 0.22 1 210
mitochondrion
10.29 0.20 93 45,791
Totals 798 599070

Change Log

Timestamp Action Category Detail
2022-09-01 Deleted DEFINITION Catalysis of the reaction: L-isoleucine + ATP + tRNA(Ile) = L-isoleucyl-tRNA(Ile) + AMP + diphosphate + 2 H(+).
2022-09-01 Added DEFINITION Catalysis of the reaction: L-isoleucine + ATP + tRNA(Ile) = L-isoleucyl-tRNA(Ile) + AMP + diphosphate + 2 H+.
2020-10-08 Added SYNONYM isoleucine-transfer RNA ligase activity
2020-10-08 Added SYNONYM L-isoleucine:tRNAIle ligase (AMP-forming)
2020-10-08 Added SYNONYM isoleucyl-transfer ribonucleate synthetase activity
2020-10-08 Deleted SYNONYM L-isoleucine:tRNAIle ligase (AMP-forming)
2020-10-08 Deleted SYNONYM isoleucine-tRNA synthetase activity
2020-10-08 Deleted SYNONYM isoleucine-transfer RNA ligase activity
2020-10-08 Deleted SYNONYM isoleucyl-transfer RNA synthetase activity
2020-10-08 Added SYNONYM isoleucyl-transfer RNA synthetase activity
Timestamp Action Category Detail
2003-03-19 Updated TERM isoleucine-tRNA ligase activity
2002-02-06 Updated TERM isoleucine-tRNA ligase
2001-11-07 Updated TERM isoleucine-tRNA ligase
2001-04-03 Updated TERM isoleucine--tRNA ligase
2001-03-30 Added TERM isoleucine--tRNA ligase
Timestamp Action Category Detail
2022-09-01 Deleted DEFINITION Catalysis of the reaction: L-isoleucine + ATP + tRNA(Ile) = L-isoleucyl-tRNA(Ile) + AMP + diphosphate + 2 H(+).
2022-09-01 Added DEFINITION Catalysis of the reaction: L-isoleucine + ATP + tRNA(Ile) = L-isoleucyl-tRNA(Ile) + AMP + diphosphate + 2 H+.
2020-10-08 Added SYNONYM isoleucine-transfer RNA ligase activity
2020-10-08 Added SYNONYM L-isoleucine:tRNAIle ligase (AMP-forming)
2020-10-08 Added SYNONYM isoleucyl-transfer ribonucleate synthetase activity
2020-10-08 Deleted SYNONYM L-isoleucine:tRNAIle ligase (AMP-forming)
2020-10-08 Deleted SYNONYM isoleucine-tRNA synthetase activity
2020-10-08 Deleted SYNONYM isoleucine-transfer RNA ligase activity
2020-10-08 Deleted SYNONYM isoleucyl-transfer RNA synthetase activity
2020-10-08 Added SYNONYM isoleucyl-transfer RNA synthetase activity
2020-10-08 Added SYNONYM isoleucine translase activity
2020-10-08 Deleted SYNONYM isoleucyl-transfer ribonucleate synthetase activity
2020-10-08 Added SYNONYM isoleucine-tRNA synthetase activity
2020-10-08 Deleted SYNONYM isoleucine translase activity
2010-10-11 Deleted DEFINITION Catalysis of the reaction: ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
2010-10-11 Added DEFINITION Catalysis of the reaction: L-isoleucine + ATP + tRNA(Ile) = L-isoleucyl-tRNA(Ile) + AMP + diphosphate + 2 H(+).
2008-05-13 Added DEFINITION Catalysis of the reaction: ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
2008-05-12 Deleted DEFINITION Catalysis of the reaction: ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
2007-11-08 Added SYNONYM isoleucyl-transfer ribonucleate synthetase activity
2007-11-08 Added SYNONYM isoleucyl-transfer RNA synthetase activity
2007-11-08 Added SYNONYM isoleucine-transfer RNA ligase activity
2007-11-08 Added SYNONYM isoleucine-tRNA synthetase activity
2007-11-08 Added SYNONYM L-isoleucine:tRNAIle ligase (AMP-forming)
2007-09-06 Added SYNONYM isoleucine translase activity
2007-08-09 Added SYNONYM isoleucyl-tRNA synthetase activity
2007-08-09 Deleted SYNONYM isoleucyl-tRNA synthetase activity
2004-04-19 Added SYNONYM isoleucyl-tRNA synthetase activity
2004-04-16 Deleted SYNONYM isoleucyl-tRNA synthetase activity
2003-12-16 Added SYNONYM isoleucyl-tRNA synthetase activity
2002-11-27 Added DEFINITION Catalysis of the reaction: ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Timestamp Action Category Detail
2008-04-01 Updated RELATION is a GO:0004812 (aminoacyl-tRNA ligase activity)
Timestamp Action Category Detail
2019-06-20 Deleted XREF KEGG:R03656
2019-06-20 Added XREF KEGG_REACTION:R03656
2019-04-01 Deleted XREF reactome:R-HSA-380176
2019-04-01 Added XREF Reactome:R-HSA-379893
2019-04-01 Added XREF Reactome:R-HSA-380176
2019-04-01 Deleted XREF reactome:R-HSA-379893
2018-12-11 Added XREF reactome:R-HSA-379893
2018-12-11 Added XREF reactome:R-HSA-380176
2018-07-13 Deleted XREF RHEA:11063
2018-07-13 Added XREF RHEA:11060
2010-10-11 Added XREF RHEA:11063
2010-10-11 Added XREF KEGG:R03656
2003-10-27 Added XREF MetaCyc:ISOLEUCINE--TRNA-LIGASE-RXN
2001-03-30 Added XREF EC:6.1.1.5
Timestamp Action Category Detail
Please Wait...

Not found :(

Sorry, but the Term you were trying to view does not exist.

Reported error:

It looks like this may have been caused by:

  • a mistyped URL
  • an out-of-date link
This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our Privacy Notice and Terms of Use.