This term is obsolete.
A protein complex in this context is meant as a stable set of interacting proteins which can be co-purified by an acceptable method, and where the complex has been shown to exist as an isolated, functional unit in vivo. Acceptable experimental methods include stringent protein purification followed by detection of protein interaction. The following methods should be considered non-acceptable: simple immunoprecipitation, pull-down experiments from cell extracts without further purification, colocalization and 2-hybrid screening. Interactions that should not be captured as protein complexes include: 1) enzyme/substrate, receptor/ligand or any similar transient interactions, unless these are a critical part of the complex assembly or are required e.g. for the receptor to be functional; 2) proteins associated in a pull-down/co-immunoprecipitation assay with no functional link or any evidence that this is a defined biological entity rather than a loose-affinity complex; 3) any complex where the only evidence is based on genetic interaction data; 4) partial complexes, where some subunits (e.g. transmembrane ones) cannot be expressed as recombinant proteins and are excluded from experiments (in this case, independent evidence is necessary to find out the composition of the full complex, if known). Interactions that may be captured as protein complexes include: 1) enzyme/substrate or receptor/ligand if the complex can only assemble and become functional in the presence of both classes of subunits; 2) complexes where one of the members has not been shown to be physically linked to the other(s), but is a homologue of, and has the same functionality as, a protein that has been experimentally demonstrated to form a complex with the other member(s); 3) complexes whose existence is accepted based on localization and pharmacological studies, but for which experimental evidence is not yet available for the complex as a whole.

GO:0032991 JSON

protein-containing complex

Cellular Component

Definition (GO:0032991 GONUTS page)

A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together.

Comments

A protein complex in this context is meant as a stable set of interacting proteins which can be co-purified by an acceptable method, and where the complex has been shown to exist as an isolated, functional unit in vivo. Acceptable experimental methods include stringent protein purification followed by detection of protein interaction. The following methods should be considered non-acceptable: simple immunoprecipitation, pull-down experiments from cell extracts without further purification, colocalization and 2-hybrid screening. Interactions that should not be captured as protein complexes include: 1) enzyme/substrate, receptor/ligand or any similar transient interactions, unless these are a critical part of the complex assembly or are required e.g. for the receptor to be functional; 2) proteins associated in a pull-down/co-immunoprecipitation assay with no functional link or any evidence that this is a defined biological entity rather than a loose-affinity complex; 3) any complex where the only evidence is based on genetic interaction data; 4) partial complexes, where some subunits (e.g. transmembrane ones) cannot be expressed as recombinant proteins and are excluded from experiments (in this case, independent evidence is necessary to find out the composition of the full complex, if known). Interactions that may be captured as protein complexes include: 1) enzyme/substrate or receptor/ligand if the complex can only assemble and become functional in the presence of both classes of subunits; 2) complexes where one of the members has not been shown to be physically linked to the other(s), but is a homologue of, and has the same functionality as, a protein that has been experimentally demonstrated to form a complex with the other member(s); 3) complexes whose existence is accepted based on localization and pharmacological studies, but for which experimental evidence is not yet available for the complex as a whole.

Secondary IDs

GO:0043234

26,794,092 annotations

Synonyms

Synonyms are alternative words or phrases closely related in meaning to the term name, with indication of the relationship between the name and synonym given by the synonym scope.

Synonym Type
protein containing complex exact
macromolecular complex exact
macromolecule complex exact
protein-protein complex narrow
protein complex narrow

Ancestor Chart

Ancestor chart for GO:0032991
Chart options
Ancestor chart

Child Terms

This table lists all terms that are direct descendants (child terms) of GO:0032991

Child Term Relationship to
GO:0032991
PYM-mago-Y14 complex
is_a
Arp2/3 protein complex
is_a
TBK1-IKKE-DDX3 complex
is_a
piccolo histone acetyltransferase complex
is_a
dimethyl sulfoxide reductase complex
is_a
ASAP complex
is_a
muscle thin filament tropomyosin
is_a
MAML1-RBP-Jkappa- ICN1 complex
is_a
PET complex
is_a
polarisome
is_a

Co-occurring Terms

These tables show the number of times the term listed in the table has been co-annotated.

No co-occurrence statistics for GO:0032991 based on ALL annotations
The top 100 of 9,801 co-occurring terms
Co-occurring Term PR S% #Together #Compared
protein-containing complex
1,481.42 100.00 133,760 133,760
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
351.34 4.61 7,232 30,494
establishment or maintenance of epithelial cell apical/basal polarity
266.20 4.39 7,339 40,842
Lsm1-7-Pat1 complex
567.22 4.12 5,903 15,417
nucleoplasm
56.84 3.25 23,582 614,589
SNARE complex assembly
781.14 3.23 4,453 8,445
lytic vacuole
942.64 3.19 4,353 6,841
U2-type prespliceosome
184.05 3.12 5,342 42,997
U5 snRNP
166.72 2.97 5,184 46,064
methionyl glutamyl tRNA synthetase complex
994.55 2.96 4,014 5,979
Totals 556002 11478979
No co-occurrence statistics for GO:0032991 based on MANUAL annotations
The top 100 of 8,510 co-occurring terms
Co-occurring Term PR S% #Together #Compared
protein-containing complex
357.44 100.00 3,949 3,949
protein stabilization
82.58 9.24 527 2,281
perinuclear region of cytoplasm
56.83 9.00 679 4,271
cellular response to heat
68.08 8.06 484 2,541
protein-containing complex binding
51.53 5.75 345 2,393
ATP binding
29.38 5.50 563 6,849
protein domain specific binding
80.63 5.27 254 1,126
identical protein binding
25.57 5.20 632 8,833
enzyme binding
55.41 4.46 233 1,503
unfolded protein binding
23.11 4.40 478 7,394
Totals 23890 437792

GO Slims

This term is present in the following GO Consortium-maintained GO slims:

GO slim name Total Number of Terms in Slim
goslim_pir 438
goslim_flybase_ribbon 44
goslim_agr 53
goslim_chembl 296
goslim_mouse 45
goslim_euk_cellular_processes_ribbon 61

Change Log

Timestamp Action Category Detail
2024-03-22 Added SUBSET goslim_euk_cellular_processes_ribbon
2022-04-05 Deleted SUBSET goslim_generic
2022-04-05 Added SUBSET goslim_mouse
2018-09-22 Added SUBSET goslim_flybase_ribbon
2018-02-18 Updated TERM protein-containing complex
2018-02-18 Added SECONDARY GO:0043234 (protein complex)
2018-02-18 Added SYNONYM protein complex
2018-02-18 Added SYNONYM macromolecular complex
2018-02-18 Added SYNONYM protein-protein complex
2018-02-18 Added SUBSET goslim_chembl
Timestamp Action Category Detail
2018-02-18 Updated TERM protein-containing complex
2007-03-09 Added TERM macromolecular complex
Timestamp Action Category Detail
2018-02-18 Added SYNONYM protein complex
2018-02-18 Added SYNONYM macromolecular complex
2018-02-18 Added SYNONYM protein-protein complex
2016-10-05 Deleted DEFINITION A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which the constituent parts function together.
2016-10-05 Added DEFINITION A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together.
2016-10-05 Added SYNONYM protein containing complex
2008-05-13 Added DEFINITION A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which the constituent parts function together.
2008-05-12 Deleted DEFINITION A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which the constituent parts function together.
2007-08-09 Deleted SYNONYM macromolecule complex
2007-08-09 Added SYNONYM macromolecule complex
2007-03-13 Added DEFINITION A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which the constituent parts function together.
2007-03-09 Added SYNONYM macromolecule complex
Timestamp Action Category Detail
2008-04-01 Updated RELATION is a GO:0005575 (cellular_component)
Timestamp Action Category Detail
Timestamp Action Category Detail
2024-03-22 Added SUBSET goslim_euk_cellular_processes_ribbon
2022-04-05 Deleted SUBSET goslim_generic
2022-04-05 Added SUBSET goslim_mouse
2018-09-22 Added SUBSET goslim_flybase_ribbon
2018-02-18 Added SECONDARY GO:0043234 (protein complex)
2018-02-18 Added SUBSET goslim_chembl
2018-02-18 Added SUBSET goslim_generic
2018-02-18 Added SUBSET goslim_agr
2017-07-28 Deleted SUBSET goslim_agr
2017-03-04 Added SUBSET goslim_agr
2013-04-18 Updated SUBSET goslim_pir
2008-06-09 Added SUBSET goslim_pir
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