A protein complex in this context is meant as a stable set of interacting proteins which can be co-purified by an acceptable method, and where the complex has been shown to exist as an isolated, functional unit in vivo. Acceptable experimental methods include stringent protein purification followed by detection of protein interaction. The following methods should be considered non-acceptable: simple immunoprecipitation, pull-down experiments from cell extracts without further purification, colocalization and 2-hybrid screening. Interactions that should not be captured as protein complexes include: 1) enzyme/substrate, receptor/ligand or any similar transient interactions, unless these are a critical part of the complex assembly or are required e.g. for the receptor to be functional; 2) proteins associated in a pull-down/co-immunoprecipitation assay with no functional link or any evidence that this is a defined biological entity rather than a loose-affinity complex; 3) any complex where the only evidence is based on genetic interaction data; 4) partial complexes, where some subunits (e.g. transmembrane ones) cannot be expressed as recombinant proteins and are excluded from experiments (in this case, independent evidence is necessary to find out the composition of the full complex, if known). Interactions that may be captured as protein complexes include: 1) enzyme/substrate or receptor/ligand if the complex can only assemble and become functional in the presence of both classes of subunits; 2) complexes where one of the members has not been shown to be physically linked to the other(s), but is a homologue of, and has the same functionality as, a protein that has been experimentally demonstrated to form a complex with the other member(s); 3) complexes whose existence is accepted based on localization and pharmacological studies, but for which experimental evidence is not yet available for the complex as a whole.
GO:0032991
JSON
protein-containing complex
Cellular Component
Definition (GO:0032991 GONUTS page)
A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together.
Comments
A protein complex in this context is meant as a stable set of interacting proteins which can be co-purified by an acceptable method, and where the complex has been shown to exist as an isolated, functional unit in vivo. Acceptable experimental methods include stringent protein purification followed by detection of protein interaction. The following methods should be considered non-acceptable: simple immunoprecipitation, pull-down experiments from cell extracts without further purification, colocalization and 2-hybrid screening. Interactions that should not be captured as protein complexes include: 1) enzyme/substrate, receptor/ligand or any similar transient interactions, unless these are a critical part of the complex assembly or are required e.g. for the receptor to be functional; 2) proteins associated in a pull-down/co-immunoprecipitation assay with no functional link or any evidence that this is a defined biological entity rather than a loose-affinity complex; 3) any complex where the only evidence is based on genetic interaction data; 4) partial complexes, where some subunits (e.g. transmembrane ones) cannot be expressed as recombinant proteins and are excluded from experiments (in this case, independent evidence is necessary to find out the composition of the full complex, if known). Interactions that may be captured as protein complexes include: 1) enzyme/substrate or receptor/ligand if the complex can only assemble and become functional in the presence of both classes of subunits; 2) complexes where one of the members has not been shown to be physically linked to the other(s), but is a homologue of, and has the same functionality as, a protein that has been experimentally demonstrated to form a complex with the other member(s); 3) complexes whose existence is accepted based on localization and pharmacological studies, but for which experimental evidence is not yet available for the complex as a whole.
Secondary IDs
GO:0043234
Synonyms
Synonyms are alternative words or phrases closely related in meaning to the term name, with indication of the relationship between the name and synonym given by the synonym scope.
Synonym | Type |
---|---|
macromolecule complex | exact |
protein containing complex | exact |
protein-protein complex | narrow |
protein complex | narrow |
macromolecular complex | exact |
Ancestor Chart
Child Terms
This table lists all terms that are direct descendants (child terms) of GO:0032991
Child Term | Relationship to GO:0032991 |
---|---|
bursicon neuropeptide hormone complex
|
is_a |
thrombospondin complex
|
is_a |
viral portal complex
|
is_a |
Par3-APC-KIF3A complex
|
is_a |
spectrin
|
is_a |
zona pellucida receptor complex
|
is_a |
ferritin complex
|
is_a |
proteasome core complex, beta-subunit complex
|
is_a |
PTEX complex
|
is_a |
HIR complex
|
is_a |
Co-occurring Terms
These tables show the number of times the term listed in the table has been co-annotated.
No co-occurrence statistics for GO:0032991 based on ALL annotations
The top 100 of 9,842 co-occurring terms
Co-occurring Term | PR | S% | #Together | #Compared |
---|---|---|---|---|
protein-containing complex
|
1,528.48 | 100.00 | 127,538 | 127,538 |
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
|
362.40 | 4.67 | 7,016 | 29,591 |
establishment or maintenance of epithelial cell apical/basal polarity
|
274.20 | 4.45 | 7,117 | 39,672 |
Lsm1-7-Pat1 complex
|
601.44 | 4.28 | 5,839 | 14,839 |
SNARE complex assembly
|
815.15 | 3.30 | 4,331 | 8,121 |
nucleoplasm
|
59.21 | 3.29 | 22,787 | 588,196 |
lytic vacuole
|
986.26 | 3.26 | 4,229 | 6,554 |
U2-type prespliceosome
|
195.33 | 3.23 | 5,277 | 41,293 |
U5 snRNP
|
174.53 | 3.06 | 5,114 | 44,786 |
methionyl glutamyl tRNA synthetase complex
|
1,026.78 | 3.02 | 3,909 | 5,819 |
Totals | 537601 | 11229698 |
No co-occurrence statistics for GO:0032991 based on MANUAL annotations
The top 100 of 8,559 co-occurring terms
Co-occurring Term | PR | S% | #Together | #Compared |
---|---|---|---|---|
protein-containing complex
|
325.14 | 100.00 | 3,973 | 3,973 |
protein stabilization
|
73.69 | 8.68 | 490 | 2,162 |
perinuclear region of cytoplasm
|
47.78 | 8.34 | 642 | 4,369 |
cellular response to heat
|
61.16 | 7.56 | 446 | 2,371 |
protein-containing complex binding
|
48.58 | 5.88 | 351 | 2,349 |
ATP binding
|
26.23 | 5.27 | 525 | 6,509 |
protein domain specific binding
|
73.35 | 5.24 | 254 | 1,126 |
identical protein binding
|
23.52 | 5.24 | 635 | 8,780 |
enzyme binding
|
50.44 | 4.44 | 233 | 1,502 |
unfolded protein binding
|
21.05 | 4.26 | 440 | 6,796 |
Totals | 23632 | 419833 |
GO Slims
This term is present in the following GO Consortium-maintained GO slims:
GO slim name | Total Number of Terms in Slim |
---|---|
goslim_pir | 439 |
goslim_flybase_ribbon | 44 |
goslim_agr | 53 |
goslim_chembl | 296 |
goslim_mouse | 45 |
goslim_euk_cellular_processes_ribbon | 61 |
Change Log
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- Term
- Term
- Definition / Synonyms
- Definition / Synonyms
- Relationships
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- Cross-references
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Timestamp | Action | Category | Detail |
---|---|---|---|
2024-03-22 | Added | SUBSET | goslim_euk_cellular_processes_ribbon |
2022-04-05 | Added | SUBSET | goslim_mouse |
2022-04-05 | Deleted | SUBSET | goslim_generic |
2018-09-22 | Added | SUBSET | goslim_flybase_ribbon |
2018-02-18 | Added | SYNONYM | protein complex |
2018-02-18 | Added | SECONDARY | GO:0043234 (protein complex) |
2018-02-18 | Added | SUBSET | goslim_generic |
2018-02-18 | Updated | TERM | protein-containing complex |
2018-02-18 | Added | SYNONYM | macromolecular complex |
2018-02-18 | Added | SUBSET | goslim_chembl |
Timestamp | Action | Category | Detail |
---|---|---|---|
2018-02-18 | Updated | TERM | protein-containing complex |
2007-03-09 | Added | TERM | macromolecular complex |
Timestamp | Action | Category | Detail |
---|---|---|---|
2018-02-18 | Added | SYNONYM | protein complex |
2018-02-18 | Added | SYNONYM | macromolecular complex |
2018-02-18 | Added | SYNONYM | protein-protein complex |
2016-10-05 | Deleted | DEFINITION | A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which the constituent parts function together. |
2016-10-05 | Added | DEFINITION | A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together. |
2016-10-05 | Added | SYNONYM | protein containing complex |
2008-05-13 | Added | DEFINITION | A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which the constituent parts function together. |
2008-05-12 | Deleted | DEFINITION | A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which the constituent parts function together. |
2007-08-09 | Added | SYNONYM | macromolecule complex |
2007-08-09 | Deleted | SYNONYM | macromolecule complex |
2007-03-13 | Added | DEFINITION | A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which the constituent parts function together. |
2007-03-09 | Added | SYNONYM | macromolecule complex |
Timestamp | Action | Category | Detail |
---|---|---|---|
2008-04-01 | Updated | RELATION | is a GO:0005575 (cellular_component) |
Timestamp | Action | Category | Detail |
---|
Timestamp | Action | Category | Detail |
---|---|---|---|
2024-03-22 | Added | SUBSET | goslim_euk_cellular_processes_ribbon |
2022-04-05 | Added | SUBSET | goslim_mouse |
2022-04-05 | Deleted | SUBSET | goslim_generic |
2018-09-22 | Added | SUBSET | goslim_flybase_ribbon |
2018-02-18 | Added | SECONDARY | GO:0043234 (protein complex) |
2018-02-18 | Added | SUBSET | goslim_generic |
2018-02-18 | Added | SUBSET | goslim_chembl |
2018-02-18 | Added | SUBSET | goslim_agr |
2017-07-28 | Deleted | SUBSET | goslim_agr |
2017-03-04 | Added | SUBSET | goslim_agr |
2013-04-18 | Updated | SUBSET | goslim_pir |
2008-06-09 | Added | SUBSET | goslim_pir |
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