ID AJ250488; SV 1; linear; mRNA; STD; VRL; 3926 BP. XX AC AJ250488; XX DT 24-APR-2000 (Rel. 63, Created) DT 13-FEB-2001 (Rel. 66, Last updated, Version 2) XX DE tobacco rattle virus cp gene, 2b gene,2c gene and 1b gene, isolate PaY4 XX KW 1B gene; 2B gene; 2C gene; cp gene; 27K protein; 33K protein; coat protein; KW transmission helper protein. XX OS Tobacco rattle virus OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; OC Martellivirales; Virgaviridae; Tobravirus. XX RN [1] RP 1-3926 RA MacFarlane S.A.; RT ; RL Submitted (22-OCT-1999) to the INSDC. RL MacFarlane S.A., Virology, Scottish Crop Research Institute, Invergowrie, RL Dundee, DD2 5DA, UNITED KINGDOM. XX RN [2] RX DOI; 10.1006/viro.2000.0677. RX PUBMED; 11162804. RA Vassilakos N., Vellios E.K., Brown E.C., Brown D.J.F., MacFarlane S.A.; RT "Tobravirus 2b protein acts in trans to facilitate transmission by RT nematodes"; RL Virology 279(2):478-487(2001). XX DR MD5; a5da4e7fa60a8b55d26b397040c5da28. DR RFAM; RF01101; TLS-PK6. XX CC 3' region is derived from RNA1 by recombination. Recombination CC junction is CC at nucleotide 3406 and replaces the RNA2 3' non-coding region with CC the CC C-terminal part of the 1b gene and the RNA1 3' non-coding region. XX FH Key Location/Qualifiers FH FT source 1..3926 FT /organism="Tobacco rattle virus" FT /chromosome="recombination of RNA1 and RNA2" FT /isolate="PaY4" FT /mol_type="mRNA" FT /country="United Kingdom:York, England" FT /db_xref="taxon:12295" FT source 1..3406 FT /organism="Tobacco rattle virus" FT /chromosome="RNA2" FT /mol_type="mRNA" FT /db_xref="taxon:12295" FT source 3407..3926 FT /organism="Tobacco rattle virus" FT /chromosome="RNA1" FT /mol_type="mRNA" FT /db_xref="taxon:12295" FT CDS 713..1345 FT /gene="cp" FT /product="coat protein" FT /function="encapsidation" FT /db_xref="GOA:Q9IW56" FT /db_xref="InterPro:IPR001337" FT /db_xref="InterPro:IPR036417" FT /db_xref="UniProtKB/TrEMBL:Q9IW56" FT /protein_id="CAB88864.1" FT /translation="MAGTYGDSFEGKILDDLSGAWVEKHNWSDVLKRLTKIKFALQADR FT DMIPGIVEDLNTEIPVDEFTRFPAGKVYHLLSKELLMAIEAIHAASSFKRRAEEKNNVN FT PRQRFDAEGSATQLNPGGTVVRPVTGNNDSSLGEDMFSNSKLDDASTAFHKSLATLKGS FT RPKAVVQRTFEREYSLKWVAGAAGTPAGVNAVAGGGTPSGGGRGPNS" FT CDS 1476..2192 FT /gene="2b" FT /product="27K transmission helper protein" FT /function="nematode transmission" FT /db_xref="GOA:Q9IW55" FT /db_xref="InterPro:IPR007935" FT /db_xref="UniProtKB/TrEMBL:Q9IW55" FT /protein_id="CAB88865.1" FT /translation="MVNGTWADKWPVENLFIDDFGKLVWFDILDDVVRITHFVSQVPTD FT LSNIPKAFISFIDNRVPMCINSKGWMFIRVKVDGEDVYYQKLGELDVSSFGDSILPPDF FT EFKFTKVTVPVDKQLVSKVNSLERLNAQLTTDLSQARTLKNFEELREGLVLNYSKAGGR FT LFRMVVRMIGGNWKLVSEINNDSDRWATTSANLVLKPDAQYLGYKVVKRFNVIGLEFDL FT ARLNAIDVPTLLASVV" FT CDS 2367..3227 FT /gene="2c" FT /product="33K protein" FT /db_xref="GOA:Q9IW54" FT /db_xref="UniProtKB/TrEMBL:Q9IW54" FT /protein_id="CAB88866.1" FT /translation="MGDVFSAFIIELTSEVTFSVGFNKKNFFTNSGRVFPFDGTIPKIG FT NWLVVYCHWWPDGSTTIHLSTRSHGVDVGKSCTFSVTGRNRRKFQVERDLPMRNTVSYR FT NVNFEDGFRIRFPESSPFTVDFVVSFRPNLCKFSNASGSIIVVGPRFDAAVGLESYLDL FT LKGRLRISRSVIPVIALVVSIISVLVSISGIVVNALTSFTPVEDVVLVWVDSIHRFVVY FT KHGRFDIQSIRTVSVEQAKALLAGPTSIEKFFREYGWKVYSPFFTALDWDQVAKDSVFG FT STYST" FT CDS <3406..3671 FT /pseudo FT /gene="1b" XX SQ Sequence 3926 BP; 1092 A; 635 C; 928 G; 1271 T; 0 other; ataaaacatt gcacctttgg tgctgccctg gctggggtgt gtctttgaac gcagtagaat 60 gtgctaattg acaagttgga gaacgcggta gaacgtactt atccgacaca gcctttatcc 120 ctcttcctga cgaggttttt gtcagtgtat catgttgttt tgaactatcc aacttagtac 180 cgaatgggga aagtgattgg tgtgcttatc ttcaatatga tgctttgaag tttgaatagt 240 aggaagttag aaaagaaact cttgtcttct caaacaagta aaacctgaga cgtgttaact 300 acgaaaatgt ccattttaaa atctgtggtt gaatgttgtg ttcaactgtg gaaaccaaaa 360 ccgacgtggg aagaactcga ctaatacttt agtctgaggt gttcctacga tcgcaagggt 420 gtgttaaagc cctgggggtt ttctgatttc tttagagaaa atcagataag atcttgatat 480 tgttattatt gaggttggca taattatact gattgtgtcc tcgttgatag agactatcat 540 ctgtcattta tctcaagaag cagtggcatt ggttcggaac gacctcattt cttagttatt 600 taactaggaa ttgcataaat ttaacaaaga ttgctaagca acttattctg tacactttga 660 taagaagatt tgctgaaaca ctggttgctt aatcacttac tcttcttcac taatggctgg 720 gacatatgga gattcctttg aaggaaagat cttagatgat ctgtctgggg cttgggtcga 780 gaagcataat tggagcgatg tccttaaaag gctgacaaag ataaagtttg ccttacaagc 840 tgatagggat atgattcctg gaatcgtgga agatttgaac actgaaattc cagtggatga 900 atttactaga ttcccggctg gtaaggttta tcacctgctg tccaaagaat tgttgatggc 960 gattgaagct attcacgctg cgtcttcttt caaaagaaga gcggaagaaa agaataatgt 1020 caatcccagg cagcgatttg atgctgaagg atctgcaacg cagttaaacc cagggggaac 1080 tgtggttaga cccgttaccg gtaacaacga ttcatctttg ggcgaagata tgttctctaa 1140 tagtaagttg gacgacgcca gtactgcttt ccataagagt ttggctacgt taaaaggcag 1200 tcggcctaaa gcggttgtcc aaagaacatt tgagagagaa tacagtctga agtgggtggc 1260 aggagcagct gggacaccgg caggtgttaa tgcggtagcc ggtggaggta ctccgtctgg 1320 tggcggacgc ggtccgaatt cttgattttc tcagctttgt cggtattcta tggtctgttt 1380 gaccatttgg tttcgatttt gaatggcgtt ggatcacgat tatgatcgca gttttaaatg 1440 tcttttgact tttgtcgcag acggcatatg tgaaaatggt taacggaact tgggctgaca 1500 aatggccagt tgaaaatttg ttcattgatg attttggaaa gttggtgtgg tttgatattc 1560 tagacgatgt ggttcgcatc actcatttcg tttctcaagt gccaacagat ctttcgaata 1620 tacctaaagc attcatctcc tttattgata atcgagtccc tatgtgtata aattccaaag 1680 gttggatgtt tataagggtt aaggtcgatg gagaagacgt ttactatcag aaattggggg 1740 aactagacgt ttcttctttc ggagattcaa tattgccgcc tgacttcgag ttcaaattta 1800 ctaaggttac agtgcctgtt gataaacagc tggttagcaa agttaactct ttagagaggt 1860 taaatgctca actgacgaca gatctcagtc aagctcgtac tcttaaaaat tttgaagaac 1920 ttcgagaagg tttagtgttg aattattcaa aagcaggagg tcggctcttc agaatggtgg 1980 taagaatgat aggtggaaat tggaagttag tttccgaaat caacaatgat tcagatagat 2040 gggccactac gagcgctaac ttggtcctta aaccggacgc acaatactta ggttacaaag 2100 ttgtaaagag atttaatgtg atcggacttg aattcgattt agcaaggttg aatgcgatag 2160 acgttccaac tttgttagcg tctgttgtgt aaatcgaaac acttatgtag gtcgatcaca 2220 tcgacggtgg cctagaccat actgggtgaa gttgtgggaa gcttcgaaaa taattctcgt 2280 gggcaaatct catttgcggt ggttgttacc ataaacaata atgtcgttgg ttgcatttaa 2340 aataattcct tttgtctgtt ggtaatatgg gagatgtatt cagtgcattt attattgaat 2400 taacttcgga agttactttc tccgtggggt tcaataagaa gaactttttc accaattctg 2460 gtcgtgtgtt tcctttcgat ggtactattc caaagatcgg taactggtta gttgtttatt 2520 gtcattggtg gcctgatggt tcaacaacaa tacatctgtc aacaaggtct cacggtgtcg 2580 acgtcggaaa gtcttgtacg ttttcagtga ccggacgtaa cagacgtaaa ttccaagtgg 2640 aaagggattt gccgatgcgt aacactgttt cttacaggaa tgtgaatttt gaggacggtt 2700 tcaggatcag atttcctgag tcatctcctt tcacagtcga ttttgttgtg tcctttagac 2760 ctaacttgtg taagttttct aacgctagtg gttctattat tgtagttgga cctaggtttg 2820 acgcggcagt tggtttagaa agttatctcg atttacttaa aggtcggttg agaatttcgc 2880 gctctgtaat acccgtaatt gcgttggttg tatctataat aagtgtactt gtgtcaatat 2940 ctgggattgt tgtgaatgca ttaacaagct ttactccggt agaggatgta gtattggtgt 3000 gggtggattc aattcataga tttgttgttt acaaacatgg aagatttgac atccaatcaa 3060 ttagaacagt atcagttgag caagcaaaag cactactagc tggtcctaca agtattgaga 3120 aattctttcg tgagtacggg tggaaggtgt attccccctt cttcactgca cttgactggg 3180 accaagtggc taaagacagt gtctttgggt cgacgtattc aacttaagca agatgtttgt 3240 ctaaggacgt tgaaatcaat cacctactga gtaatttctt acctggaaaa ctgtttacgc 3300 agttagtaaa gatagaaagg aaagttgact ggggttgatt ttgttgagaa aatttgttag 3360 tatttatgtt aatcacttga tttctctgtt gtgattatag ttagtttatc aataatttta 3420 cttttggtgt gtacaattgt tgtagccgtg gtcaccttta aaaatgtcgt aaacgcgttg 3480 aagcaagaaa tcgaggaatc taggaacaga ttcgacgagg tcaagctgaa aacatatgtt 3540 cgacacctta aaagtctcat aattcgaaga cctctaagaa gaaattcaaa ggggacaaag 3600 aatttgggac accaaaaaga ttttaaagag atgatattcc tttagggatt gatcaattat 3660 ttactttttg attttatttt aaattgttat ctgtttctgt gtatagactg tttgagattg 3720 gcgtttggcc gactcattgt cttaccatag gggaatggac tttgtttgtg ttgttatttt 3780 atttgtattt tattaaaatt ctcaatgatc tgaaaaagcc tcgtggctaa gagattattg 3840 gggggtgagt aagtactttt aaagtgatga tggttacaaa ggcaaaaggg gtaaaacccc 3900 tcgcctacgt aagcgttatt acgccc 3926 //