Villanova2017 - Mixotrophic metabolism in the model diatom Phaeodactylum tricornutum

Model Identifier

MODEL2102080001

Short description

Genome-scale metabolic model of Phaeodactylum tricornutum

Format

SBML (L3V1)

Related Publication

Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum
Valeria Villanova, Antonio Emidio Fortunato, Dipali Singh, Davide Dal Bo, Melissa Conte, Toshihiro Obata, Juliette Jouhet, Alisdair R. Fernie, Eric Marechal, Angela Falciatore, Julien Pagliardini, Adeline Le Monnier, Mark Poolman, Gilles Curien, Dimitris Petroutsos, Giovanni Finazzi
Philosophical Transactions of the Royal Society B: Biological Sciences , 7/ 2017 , Volume 372 , Issue 1728 , pages: 20160404 , DOI: 10.1098/rstb.2016.0404

Affiliation: 1. Fermentalg SA, 33500 Libourne, France 2. Laboratoire de Physiologie Cellulaire et Ve´ge´tale, Universite´ Grenoble Alpes (UGA), Centre National de la Recherche Scientifique (CNRS), Commissariat a` l’Energie Atomique et aux Energies Alternatives (CEA), Institut National Recherche Agronomique (INRA), Institut de Biosciences et Biotechnologies de Grenoble (BIG), CEA Grenoble, F-38000 Grenoble, France 3. Laboratoire de Biologie Computationnelle et Quantitative, Sorbonne Universite´s, UPMC, Institut de Biologie Paris-Seine, CNRS, 15 rue de l’Ecole de Me´decine, Paris 75006, France 4. Max-Planck Institut fu¨r Molekulare Pflanzenphysiologie, Am Mu¨hlenberg 1, 14476 Golm-Potsdam, Germany 5. Department of Biological and Medical Sciences, Oxford Brookes University, Oxford OX3 0BP, UK

Abstract: Diatoms are prominent marine microalgae, interesting not only from an ecological point of view, but also for their possible use in biotechnology applications. They can be cultivated in phototrophic conditions, using sunlight as the sole energy source. Some diatoms, however, can also grow in a mixotrophic mode, wherein both light and external reduced carbon contribute to biomass accumulation. In this study, we investigated the consequences of mixotrophy on the growth and metabolism of the pennate diatom Phaeodactylum tricornutum, using glycerol as the source of reduced carbon. Transcriptomics, metabolomics, metabolic modelling and physiological data combine to indicate that glycerol affects the central-carbon, carbon-storage and lipid metabolism of the diatom. In particular, provision of glycerol mimics typical responses of nitrogen limitation on lipid metabolism at the level of triacylglycerol accumulation and fatty acid composition. The presence of glycerol, despite provoking features reminiscent of nutrient limitation, neither diminishes photosynthetic activity nor cell growth, revealing essential aspects of the metabolic flexibility of these microalgae and suggesting possible biotechnological applications of mixotrophy.

Contributors

Submitter of the first revision: Dipali
Submitter of this revision: Krishna Kumar Tiwari
Modellers: Dipali, Krishna Kumar Tiwari

Metadata information

hasProperty (1 statement)

Mathematical Modelling Ontology Constraint-based model

Curation status

Non-curated

Modelling approach(es)

constraint-based model

Connected external resources

SBGN view in Newt Editor

Name	Description	Size	Actions
Model files (1)
Phaeo.sbml	Genome-scale metabolic model of Phaeodactylum tricornutum	498.72 KB	Preview \| Download

Model originally submitted by : Dipali
Submitted: Mar 15, 2021 7:18:32 AM
Last Modified: Mar 15, 2021 7:18:32 AM

Revisions

Version: 3
- Submitted on: Mar 15, 2021 7:18:32 AM
- Submitted by: Krishna Kumar Tiwari
- With comment: Updated model name and authors details