# characteristics.INSDC center name[].text
University of Helsinki, Genome-Scale Biology Program

# characteristics.description[].text
Protocols: Drosophila S2 cells were cultured at controlled temperature (+23.5C; Binder KB cooling incubator) in Invitrogen SFM medium supplemented with 10% fetal bovine serum, 10% IMS (insect medium supplement, Sigma), 2 mM glutamine, and antibiotics. For transfections in 6-well plates, 1.000.000 S2 cells were seeded per well in 2 ml medium. The following day, cells were transfected with dsRNA according to manufacturers (Qiagen) instructions, using 5ul Effectene, 8.8 ul Enhancer, 1100 ng dsRNA. RNA extraction, was performed with Qiagen RNeasy kits according to manufacturers instruction, including the additional DNase treatment.