SAMEA2697775
ERS525831
E-MTAB-2684:si_p60_B
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Attributes
Type |
Value |
ENA-CHECKLIST |
ERC000011 |
ENA-FIRST-PUBLIC |
2015-08-02T17:01:19Z |
ENA-LAST-UPDATE |
2018-03-08T19:31:17Z |
External Id |
SAMEA2697775 |
INSDC center name |
Max Plank Institute for Molecular Biomedicine |
INSDC first public |
2015-08-02T17:01:19Z |
INSDC last update |
2018-03-08T19:31:17Z |
INSDC status |
public |
SRA accession |
ERS525831 |
Submitter Id |
E-MTAB-2684:si_p60_B |
broker name |
ArrayExpress |
cell line |
ES-E14 cell |
cell type |
embryonic stem cell |
common name |
house mouse |
description |
Protocols: Total RNA was extracted with GenElute total RNA miniprep kit, treated with turbo DNase and purified with RNeasy MinElute Cleanup kit. The quality was assessed on a bioanalyzer. ES cells were cultured in DMEM with GlutaMax (Life technologies) containing 15% FCS and LIF Libraries were prepared with Truseq Stranded Total RNA with Ribo-Zero Gold Prep kit (Illumina). Lipofectamine RNAi MAX (Life technologies) were used to transfect siRNA. |
genotype |
wild type genotype |
organism |
Mus musculus |
organism |
Mus musculus |
scientific_name |
Mus musculus |
strain |
129/Ola |
title |
si_p60_B |
BioSamples record history
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