SAMEA2697775

ERS525831

E-MTAB-2684:si_p60_B

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Attributes

Type Value
ENA-CHECKLIST ERC000011
ENA-FIRST-PUBLIC 2015-08-02T17:01:19Z
ENA-LAST-UPDATE 2018-03-08T19:31:17Z
External Id SAMEA2697775
INSDC center name Max Plank Institute for Molecular Biomedicine
INSDC first public 2015-08-02T17:01:19Z
INSDC last update 2018-03-08T19:31:17Z
INSDC status public
SRA accession ERS525831
Submitter Id E-MTAB-2684:si_p60_B
broker name ArrayExpress
cell line ES-E14 cell
cell type embryonic stem cell
common name house mouse
description Protocols: Total RNA was extracted with GenElute total RNA miniprep kit, treated with turbo DNase and purified with RNeasy MinElute Cleanup kit. The quality was assessed on a bioanalyzer. ES cells were cultured in DMEM with GlutaMax (Life technologies) containing 15% FCS and LIF Libraries were prepared with Truseq Stranded Total RNA with Ribo-Zero Gold Prep kit (Illumina). Lipofectamine RNAi MAX (Life technologies) were used to transfect siRNA.
genotype wild type genotype
organism Mus musculus
organism Mus musculus
scientific_name Mus musculus
strain 129/Ola
title si_p60_B

Relationships

Source Type Target
SAMEG302790 has member SAMEA2697775

External Links

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BioSamples record history

Submitted on 2014-08-12
Released on 2015-08-02
Last reviewed 2024-02-05
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