SAMEA6864906
ERS4592481
Run201_BC18
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Attributes
| Type |
Value |
| ENA-CHECKLIST |
ERC000033 |
| ENA-FIRST-PUBLIC |
2020-05-30T04:06:06Z |
| ENA-LAST-UPDATE |
2022-06-12T00:43:24Z |
| External Id |
SAMEA6864906 |
| GISAID Accession ID |
EPI_ISL_422756 |
| INSDC center name |
ERASMUS MC, UNIVERISTY MEDICAL CENTER |
| INSDC first public |
2020-05-30T04:06:06Z |
| INSDC last update |
2022-06-12T00:43:24Z |
| INSDC status |
public |
| SRA accession |
ERS4592481 |
| Submitter Id |
Run201_BC18 |
| broker name |
ERASMUS MC, UNIVERISTY MEDICAL CENTER |
| collecting institution |
not provided |
| collection date |
2020-03-27 |
| collector name |
Dutch COVID-19 response team |
| common name |
Human coronavirus 2019 |
| description |
A SARS-CoV-2 specific multiplex PCR for Nanopore sequencing was performed, similar to amplicon-based approaches as previously described. In short, primers for 86 overlapping amplicons spanning the entire genome were designed using primal. The amplicon length was set to 500bp with 75bp overlap between the different amplicons. The libraries were generated using the native barcode kits from Nanopore (EXP-NBD104 and EXP-NBD114 and SQK-LSK109) and sequenced on a R9.4 flow cell multiplexing up to 24 samples per sequence run. Raw data was demultiplexed, amplicon primers were trimmed and human data was removed by mapping against the human reference genome. |
| geographic location (country and/or sea) |
Netherlands |
| host common name |
Human |
| host scientific name |
Homo sapiens |
| host sex |
not provided |
| host subject id |
restricted access |
| isolate |
hCov-19/Netherlands/NA_221/2020 |
| organism |
Severe acute respiratory syndrome coronavirus 2 |
| project name |
ReCoDID COVID-19 pilot study |
| sample capture status |
active surveillance in response to outbreak |
| sample derived from |
SAMEA12928737 |
| scientific_name |
Severe acute respiratory syndrome coronavirus 2 |
| title |
Dutch COVID-19 response sample sequencing |
BioSamples record history
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