InChI=1S/C15H24N2O2/c18-14-6-3-5-13-11-8-10(9-17(13)14)12-4-1-2-7-16(12)15(11)19/h10-13,15,19H,1-9H2/t10-,11+,12-,13+,15?/m0/s1 |
LCORZQTZVFOPGT-IZADBBIGSA-N |
[H][C@@]12CN3C(=O)CCC[C@]3([H])[C@@]([H])(C1)C(O)N1CCCC[C@@]21[H] |
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Bronsted base
A molecular entity capable of accepting a hydron from a donor (Bronsted acid).
(via organic amino compound )
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metabolite
Any intermediate or product resulting from metabolism. The term 'metabolite' subsumes the classes commonly known as primary and secondary metabolites.
(via alkaloid )
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View more via ChEBI Ontology
15043-17-1
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CAS Registry Number
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ChemIDplus
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5959682
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Reaxys Registry Number
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Reaxys
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Hopper DJ, Rogozinski J, Toczko M (1991) Lupanine hydroxylase, a quinocytochrome c from an alkaloid-degrading Pseudomonas sp. The Biochemical journal 279 ( Pt 1), 105-109 [PubMed:1656935] [show Abstract] Lupanine 17-hydroxylase, the first enzyme in the pathway for bacterial degradation of the alkaloid, lupanine, was purified from a Pseudomonas sp. The enzyme acts by initial dehydrogenation of the substrate, and cytochrome c was used as electron acceptor in assays. It had an Mr of 66,000 by ultracentrifuge studies and 74,000 by gel filtration. The visible absorption spectrum was that of a cytochrome c, and a stoicheiometry of one haem group per molecule of enzyme was calculated. SDS/PAGE gave a single band of Mr 72,000 containing the haem group. The enzyme also contained pyrroloquinoline quinone (PQQ), which could be removed by isoelectric focusing. The apoenzyme was reconstituted to full activity with addition of PQQ, and a stoicheiometry of one molecule of PQQ per molecule of enzyme was calculated. Steady-state kinetics gave values of 3.6 microM for the Km for lupanine, 21.3 microM for the Km for cytochrome c and 217 s-1 for the Kcat. | Mazur M, Polakowski P, Szadowska A (1966) [Pharmacologic studies on 17-oxolupanine, lupanine aminooxide and 17-hydroxylupanine]. Acta physiologica Polonica 17, 311-320 [PubMed:5945524] |
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