InChI=1S/C27H35N3.2ClH/c1-20-19-23(13-18-26(20)29(4)5)27(21-9-14-24(15-10-21)28(2)3)22-11-16-25(17-12-22)30(6,7)8;;/h9-19H,1-8H3;2*1H/q+2;;/p-2 |
FRCAGVUKJQCWBD-UHFFFAOYSA-L |
C(C=1C=CC([N+](C)(C)C)=CC1)(C2=CC(=C(N(C)C)C=C2)C)=C3C=CC(=[N+](C)C)C=C3.[Cl-].[Cl-] |
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fluorochrome
A fluorescent dye used to stain biological specimens.
histological dye
A dye used in microscopic or electron microscopic examination of cells and tissues to give contrast and to highlight particular features of interest, such as nuclei and cytoplasm.
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View more via ChEBI Ontology
(4-{[4-(dimethylamino)-3-methylphenyl][4-(trimethylazaniumyl)phenyl]methylidene}cyclohexa-2,5-dien-1-ylidene)(dimethyl)ammonium dichloride
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33231-00-4
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CAS Registry Number
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ChemIDplus
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Gong Z, Zhu M, Zheng J, Zhan R, Lin W (2003) [Ziehl-Neelsen-Victoria blue-iodine green compound staining method showing leprotic bacillus]. Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences 35, 85-86 [PubMed:12920817] [show Abstract]
ObjectiveTo compare compound staining methods showing leprotic bacillus, elastic fibre and cell nucleus etc. in skin biopsy tissue.MethodsA compound staining dye composed of Ziehl-Neelsen Phenol Fuchsin, Victoria blue, iodine green and Martius yellow were used in paraffin-embedding skin biopsy tissue.ResultsLeprotic bacilli showed red; cell nuclei, light green; cell cytoplasm, colorless; elastic fibres around tuberculoid nodules, blue; and essential substances, light yellow in skin biopsy tissue.ConclusionBecause the color was single in Ziehl-Neelsen staining method, its contrast was poor. The ZN-VB-IG compound staining method may show leprotic bacilli, elastic fibres and cell nuclei etc. distinctly. Therefore, this is a reliable and nice color contrast compound staining method. | Dutt MK (1975) Selective staining of animal chromosomes with synthetic dyes following iodine-dye-procedure. Acta histochemica 53, 211-216 [PubMed:52989] [show Abstract] The paper embodies results of the use of 51 synthetic dyes, belonging to different chemical groups for staining of animal chromosomes following iodine-dye procedure. It has been found that some of these dyes can replace gentian violet, crystal violet and safranin when used after this procedure. It has further been found that the fluorescent dyes, acriflavine and acridine yellow can also be used to stain animal chromosomes and that some of the dyes belonging to one chemical group can be successfully used whereas others of the same group are of no use. Dyes of the monoazo group are absolutely useless. Amongst the dyes successfully used, the preparations remain stable when stained with most of them except methyl green, malachite green, brillant green, iodine green and cresyl violet and amongst acid dyes, acid fuchsin. Cytochemical studies presented herein indicate that the components of the animal chromosomes stainable with crystal violet are the nucleic acids and that these substances should be highly polymerised and should not be even in a semi-degraded state. Removal of any one of these nucleic acids makes the chromosomes unstainable with iodine-crystal violet. |
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