CHEBI:75422 - iodonitrotetrazolium

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ChEBI Name iodonitrotetrazolium
ChEBI ID CHEBI:75422
Definition An organic cation that is the cationic component of iodonitrotetrazolium violet.
Stars This entity has been manually annotated by the ChEBI Team.
Supplier Information No supplier information found for this compound.
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INT (iodonitrotetrazolium or 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium) is a commonly used tetrazolium salt (usually prepared with chloride ions), similar to tetrazolium chloride that on reduction produces a red formazan dye that can be used for quantitative redox assays. It is also toxic to prokaryotes. INT is an artificial electron acceptor that can be utilized in a colorimetric assay to determine the concentration of protein in a solution. It can be reduced by succinate dehydrogenase to furazan, the formation of which can be measured by absorbance at 490 nm. The activity of succinate dehydrogenase is readily observed by the naked eye as the solution turns from colorless to rusty red.
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Formula C19H13IN5O2
Net Charge +1
Average Mass 470.24330
Monoisotopic Mass 470.01085
InChI InChI=1S/C19H13IN5O2/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27/h1-13H/q+1
InChIKey YCMNFTXISHKEBZ-UHFFFAOYSA-N
SMILES [O-][N+](=O)c1ccc(cc1)-n1nc(n[n+]1-c1ccc(I)cc1)-c1ccccc1
ChEBI Ontology
Outgoing iodonitrotetrazolium (CHEBI:75422) is a organic cation (CHEBI:25697)
Incoming iodonitrotetrazolium chloride (CHEBI:75421) has part iodonitrotetrazolium (CHEBI:75422)
IUPAC Name
3-(4-iodophenyl)-2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium
Synonyms Sources
iodonitrotetrazolium purple ChEBI
iodonitrotetrazolium violet ChEBI
Manual Xrefs Databases
CPD-10339 MetaCyc
Iodonitrotetrazolium Wikipedia
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Registry Number Type Source
3722288 Reaxys Registry Number Reaxys
Last Modified
02 September 2013