SAGA complex

ComplexAc: CPX-656

Saccharomyces cerevisiae; 559292

Curated by:

IntAct

Components

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ComplexViewer 2.2.4by Rappsilber LaboratorySPT3CHD1UBP8SGF29TRA1ADA2TAF9SPT7GCN5SGF73SUS1SGF11TAF6TAF10SPT20SPT8TAF12NGG1HFI1TAF5tooltip
Legend Description Stoichiometry
protein - SUS1 (unspecified role)
Transcription and mRNA export factor SUS1
protein - SPT20 (unspecified role)
Transcription factor SPT20
protein - SPT3 (unspecified role)
Protein SPT3
protein - SPT7 (unspecified role)
Transcriptional activator SPT7
protein - SPT8 (unspecified role)
Transcription factor SPT8
Show all components

Function

A transcriptional co-activator complex with histone acetyltransferase activity. Recruited to gene loci by the interaction of TRA1 with specific transcriptional activators, and the bromodomain of GCN5 binds acetylated H3 and H4 N-terminal tails which potentiates cooperative nucleosome acetylation of histone H3 catalysed by the acetylation module. This opens up the chromatin landscape for binding of additional transcription factors and the pre-initiation complex. The DUB module facilitates elongation through deubiquitination of H2B which allows for the recruitment of the CTK1 kinase (Q03957) and subsequent Ser-2 phosophorylation of the Pol II C-terminal domain.

Properties

Organized into several functional submodules: a histone acetyltransferase (HAT) module consisting of GCN5, ADA2, NGG1/ADA3 and SGF29, a histone deubiquitinase (DUB) module consisting of UBP8, SGF11, SGF73 and SUS1, a core architecture module consisting of HFI1, SPT7, SPT20, TAF5, TAF6, TAF9, TAF10 and TAF12, a TBP-interaction module consisting of SPT3 and SPT8, substrate recognition subunit CHD1 and transcription factor-binding subunit TRA1. Closely related to SAGA-like SLIK complex (CPX-675) but contains additional subunits SPT8, SGF11, SGF73 and SUS1 and lacks RTG2 (P32608). MW = 1.8 MDa.

Expression and Cellular Location

Gene Expression Map

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GO - Cellular Component (1)

SAGA complex

Additional Information

Further Reading

Identifier Title Author(s)
10422263 Chromatin and transcription in Saccharomyces cerevisiae. Pérez-Martín J.
15260971 Molecular architecture of the S. cerevisiae SAGA complex. Wu PY, Ruhlmann C, Winston F, Schultz P.
12186975 SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription. Sterner DE, Belotserkovskaya R, Berger SL.
15647753 Chd1 chromodomain links histone H3 methylation with SAGA- and SLIK-dependent acetylation. Pray-Grant MG, Daniel JA, Schieltz D, Yates JR, Grant PA.
14718168 Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated mRNA export machinery. Rodríguez-Navarro S, Fischer T, Luo MJ, Antúnez O, Brettschneider S, Lechner J, Pérez-Ortín JE, Reed R, Hurt E.
15506919 Proteomic analysis of chromatin-modifying complexes in Saccharomyces cerevisiae identifies novel subunits. Lee KK, Prochasson P, Florens L, Swanson SK, Washburn MP, Workman JL.
20434206 Structural basis for assembly and activation of the heterotetrameric SAGA histone H2B deubiquitinase module. Köhler A, Zimmerman E, Schneider M, Hurt E, Zheng N.
25216679 Architecture of the Saccharomyces cerevisiae SAGA transcription coactivator complex. Han Y, Luo J, Ranish J, Hahn S.

Synonyms (3)

Spt-Ada-Gcn5 acetyltransferase complex
ADA2-ADA3-CHD1-GCN5-ADA1-SGF11-SGF29-SGF73-ADA5-SPT3-SPT7-SPT8-SUS1-TAF10-TAF12-TAF5-TAF6-TAF9-TRA1-UBP8 complex
Something about silencing complex

Systematic Name

ADA2:ADA3:CHD1:GCN5:HFI1:SGF11:SGF29:SGF73:SPT20:SPT3:SPT7:SPT8:SUS1:TAF10:TAF12:TAF5:TAF6:TAF9:TRA1:UBP8