SAGA complex
Components
Legend | Description | Stoichiometry | |||
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protein -
SUS1 (unspecified role)
Transcription and mRNA export factor SUS1
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protein -
SPT20 (unspecified role)
Transcription factor SPT20
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protein -
SPT3 (unspecified role)
Protein SPT3
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protein -
SPT7 (unspecified role)
Transcriptional activator SPT7
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protein -
SPT8 (unspecified role)
Transcription factor SPT8
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Show all components |
Function
A transcriptional co-activator complex with histone acetyltransferase activity. Recruited to gene loci by the interaction of TRA1 with specific transcriptional activators, and the bromodomain of GCN5 binds acetylated H3 and H4 N-terminal tails which potentiates cooperative nucleosome acetylation of histone H3 catalysed by the acetylation module. This opens up the chromatin landscape for binding of additional transcription factors and the pre-initiation complex. The DUB module facilitates elongation through deubiquitination of H2B which allows for the recruitment of the CTK1 kinase (Q03957) and subsequent Ser-2 phosophorylation of the Pol II C-terminal domain.
Catalytic Activity
GO - Molecular Function (4)
histone binding |
histone acetyltransferase activity |
thiol-dependent ubiquitinyl hydrolase activity |
nucleosomal DNA binding |
GO - Biological Process (1)
regulation of transcription from RNA polymerase II promoter |
Properties
3D Structure
Curated structures (7):
Identifier | Qualifier | |
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3kjl |
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3kik |
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3M99 |
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3MHH |
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4FIP |
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Additional Information
Further Reading
Identifier | Title | Author(s) |
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10422263 | Chromatin and transcription in Saccharomyces cerevisiae. | Pérez-Martín J. |
15260971 | Molecular architecture of the S. cerevisiae SAGA complex. | Wu PY, Ruhlmann C, Winston F, Schultz P. |
12186975 | SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription. | Sterner DE, Belotserkovskaya R, Berger SL. |
15647753 | Chd1 chromodomain links histone H3 methylation with SAGA- and SLIK-dependent acetylation. | Pray-Grant MG, Daniel JA, Schieltz D, Yates JR, Grant PA. |
14718168 | Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated mRNA export machinery. | Rodríguez-Navarro S, Fischer T, Luo MJ, Antúnez O, Brettschneider S, Lechner J, Pérez-Ortín JE, Reed R, Hurt E. |
15506919 | Proteomic analysis of chromatin-modifying complexes in Saccharomyces cerevisiae identifies novel subunits. | Lee KK, Prochasson P, Florens L, Swanson SK, Washburn MP, Workman JL. |
20434206 | Structural basis for assembly and activation of the heterotetrameric SAGA histone H2B deubiquitinase module. | Köhler A, Zimmerman E, Schneider M, Hurt E, Zheng N. |
25216679 | Architecture of the Saccharomyces cerevisiae SAGA transcription coactivator complex. | Han Y, Luo J, Ranish J, Hahn S. |
Synonyms (3)
Spt-Ada-Gcn5 acetyltransferase complex |
ADA2-ADA3-CHD1-GCN5-ADA1-SGF11-SGF29-SGF73-ADA5-SPT3-SPT7-SPT8-SUS1-TAF10-TAF12-TAF5-TAF6-TAF9-TRA1-UBP8 complex |
Something about silencing complex |
Systematic Name
ADA2:ADA3:CHD1:GCN5:HFI1:SGF11:SGF29:SGF73:SPT20:SPT3:SPT7:SPT8:SUS1:TAF10:TAF12:TAF5:TAF6:TAF9:TRA1:UBP8 |