Anaphase-promoting complex, slp1 variant

ComplexAc: CPX-764

Schizosaccharomyces pombe (strain ATCC 38366 / 972); 284812

Curated by:

IntAct

Components

  • Collapse
  • Scale:
ComplexViewer 2.2.4by Rappsilber Laboratoryapc11apc2cut4cut20nuc2apc10hcn1slp1apc13apc5cut23apc15apc14cut9tooltip
Legend Description Stoichiometry
protein - cut23 (unspecified role)
Anaphase-promoting complex subunit 8
protein - cut4 (unspecified role)
Anaphase-promoting complex subunit 1
protein - cut9 (unspecified role)
Anaphase-promoting complex subunit cut9
protein - apc2 (unspecified role)
Anaphase-promoting complex subunit 2
protein - nuc2 (unspecified role)
Anaphase-promoting complex subunit 3
Show all components

Function

APC, a key regulator of cell cycle progression, is a conserved E3 ubiqutin ligase which facilitates multiubiqutination of cell cycle proteins, including cyclins, so marking them for proteasomal destruction. Generally believed that APC acts as a scaffold that brings E2 enzymes and substrates into close proximity. APC activity is required for metaphase-anaphase transition, mitotic exit, G1 phase and DNA replication. Targets securin for destruction, which is necessary for chromosome segregation. APC seems to have similar function in meiosis. Co-activators, Slp1 and Srw1/Ste9, associate with APC core (CPX-763) complex at specific stages of cell cycle, and are thought to be involved in substrate specificity. APC-Slp1 is active in presence of high cyclin-cdk activity in M phase but after metaphase when cyclin-cdk activity decreases, Srw1/Ste9 is dephosphorylated, Slp1 is degraded and APC-Srw1/Ste9 (CPX-765) activated. Mfr1/Fzr1 (CPX-766) is meiotic co-activator required for sporulation. All APC co-activators, characterized by the presence of sequence elements, C-box and the IR-tail, that mediate their binding to APC, contain a C-terminal WD40 domain, predicted to fold into a propeller-like structure, believed to recognize APC substrates by interacting with specific recognition elements in substrates, D-boxes and KEN-boxes. Genetic inactivation of APC is lethal.

Catalytic Activity

EC Numbers (1)

6.3.2.19

Properties

Contains subunits with cullin and RING-finger domains.

Expression and Cellular Location

Gene Expression Map

Loading...

GO - Cellular Component (1)

anaphase-promoting complex

Additional Information

Further Reading

Identifier Title Author(s)
9695848 Proteolytic ratchets that control progression through mitosis. Townsley FM, Ruderman JV.
15678131 The anaphase-promoting complex: a key factor in the regulation of cell cycle. Castro A, Bernis C, Vigneron S, Labbé JC, Lorca T.
16896351 The anaphase promoting complex/cyclosome: a machine designed to destroy. Peters JM.
16364911 Structural analysis of the anaphase-promoting complex reveals multiple active sites and insights into polyubiquitylation. Passmore LA, Booth CR, Vénien-Bryan C, Ludtke SJ, Fioretto C, Johnson LN, Chiu W, Barford D.
10921876 APC(ste9/srw1) promotes degradation of mitotic cyclins in G(1) and is inhibited by cdc2 phosphorylation. Blanco MA, Sánchez-Díaz A, de Prada JM, Moreno S.
15797379 The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1. Oelschlaegel T, Schwickart M, Matos J, Bogdanova A, Camasses A, Havlis J, Shevchenko A, Zachariae W.

Synonyms (6)

apc1-apc2-apc3-apc4-apc5-apc8-apc10-apc11-apc13-apc14-apc15-cut9-hcn1-slp1 complex
Anaphase-Promoting Complex
Anaphase Promoting Complex/Cyclosome
APC/C
APC
Show all

Systematic Name

cut4:apc2:nuc2:cut20:apc5:cut23:apc10:apc11:apc13:apc14:apc15:cut9:hcn1:slp1