Anaphase-promoting complex, slp1 variant
Components
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protein -
cut23 (unspecified role)
Anaphase-promoting complex subunit 8
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protein -
cut4 (unspecified role)
Anaphase-promoting complex subunit 1
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protein -
cut9 (unspecified role)
Anaphase-promoting complex subunit cut9
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protein -
apc2 (unspecified role)
Anaphase-promoting complex subunit 2
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protein -
nuc2 (unspecified role)
Anaphase-promoting complex subunit 3
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Function
APC, a key regulator of cell cycle progression, is a conserved E3 ubiqutin ligase which facilitates multiubiqutination of cell cycle proteins, including cyclins, so marking them for proteasomal destruction. Generally believed that APC acts as a scaffold that brings E2 enzymes and substrates into close proximity. APC activity is required for metaphase-anaphase transition, mitotic exit, G1 phase and DNA replication. Targets securin for destruction, which is necessary for chromosome segregation. APC seems to have similar function in meiosis. Co-activators, Slp1 and Srw1/Ste9, associate with APC core (CPX-763) complex at specific stages of cell cycle, and are thought to be involved in substrate specificity. APC-Slp1 is active in presence of high cyclin-cdk activity in M phase but after metaphase when cyclin-cdk activity decreases, Srw1/Ste9 is dephosphorylated, Slp1 is degraded and APC-Srw1/Ste9 (CPX-765) activated. Mfr1/Fzr1 (CPX-766) is meiotic co-activator required for sporulation. All APC co-activators, characterized by the presence of sequence elements, C-box and the IR-tail, that mediate their binding to APC, contain a C-terminal WD40 domain, predicted to fold into a propeller-like structure, believed to recognize APC substrates by interacting with specific recognition elements in substrates, D-boxes and KEN-boxes. Genetic inactivation of APC is lethal.
Catalytic Activity
EC Numbers (1)
6.3.2.19 |
Properties
Expression and Cellular Location
Gene Expression Map
GO - Cellular Component (1)
anaphase-promoting complex |
Additional Information
Further Reading
Identifier | Title | Author(s) |
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9695848 | Proteolytic ratchets that control progression through mitosis. | Townsley FM, Ruderman JV. |
15678131 | The anaphase-promoting complex: a key factor in the regulation of cell cycle. | Castro A, Bernis C, Vigneron S, Labbé JC, Lorca T. |
16896351 | The anaphase promoting complex/cyclosome: a machine designed to destroy. | Peters JM. |
16364911 | Structural analysis of the anaphase-promoting complex reveals multiple active sites and insights into polyubiquitylation. | Passmore LA, Booth CR, Vénien-Bryan C, Ludtke SJ, Fioretto C, Johnson LN, Chiu W, Barford D. |
10921876 | APC(ste9/srw1) promotes degradation of mitotic cyclins in G(1) and is inhibited by cdc2 phosphorylation. | Blanco MA, Sánchez-Díaz A, de Prada JM, Moreno S. |
15797379 | The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1. | Oelschlaegel T, Schwickart M, Matos J, Bogdanova A, Camasses A, Havlis J, Shevchenko A, Zachariae W. |
Synonyms (6)
apc1-apc2-apc3-apc4-apc5-apc8-apc10-apc11-apc13-apc14-apc15-cut9-hcn1-slp1 complex |
Anaphase-Promoting Complex |
Anaphase Promoting Complex/Cyclosome |
APC/C |
APC |
Show all |
Systematic Name
cut4:apc2:nuc2:cut20:apc5:cut23:apc10:apc11:apc13:apc14:apc15:cut9:hcn1:slp1 |