EMD-0688

Single-particle
3.7 Å
EMD-0688 Deposition: 15/01/2019
Map released: 20/03/2019
Last modified: 13/11/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-0688

Reconstitution and structure of a plant NLR resistosome conferring immunity

EMD-0688

Single-particle
3.7 Å
EMD-0688 Deposition: 15/01/2019
Map released: 20/03/2019
Last modified: 13/11/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Arabidopsis thaliana, Arabidopsis
Sample: resistosome
Fitted models: 6j6i (Avg. Q-score: 0.421)

Deposition Authors: Wang JZ, Wang J
Reconstitution and structure of a plant NLR resistosome conferring immunity.
Wang J , Hu M , Wang J , Qi J , Han Z , Wang G , Qi Y , Wang HW , Zhou JM , Chai J
(2019) Science , 364
PUBMED: 30948527
DOI: doi:10.1126/science.aav5870
ISSN: 1095-9203
ASTM: SCIEAS
Abstract:
Nucleotide-binding, leucine-rich repeat receptors (NLRs) perceive pathogen effectors to trigger plant immunity. Biochemical mechanisms underlying plant NLR activation have until now remained poorly understood. We reconstituted an active complex containing the Arabidopsis coiled-coil NLR ZAR1, the pseudokinase RKS1, uridylated protein kinase PBL2, and 2'-deoxyadenosine 5'-triphosphate (dATP), demonstrating the oligomerization of the complex during immune activation. The cryo-electron microscopy structure reveals a wheel-like pentameric ZAR1 resistosome. Besides the nucleotide-binding domain, the coiled-coil domain of ZAR1 also contributes to resistosome pentamerization by forming an α-helical barrel that interacts with the leucine-rich repeat and winged-helix domains. Structural remodeling and fold switching during activation release the very N-terminal amphipathic α helix of ZAR1 to form a funnel-shaped structure that is required for the plasma membrane association, cell death triggering, and disease resistance, offering clues to the biochemical function of a plant resistosome.