EMD-11483

Single-particle
9.4 Å
EMD-11483 Deposition: 27/07/2020
Map released: 04/08/2021
Last modified: 10/07/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-11483

C17 symmetry: Bacterial Vipp1 and PspA are members of the ancient ESCRT-III membrane-remodeling superfamily.

EMD-11483

Single-particle
9.4 Å
EMD-11483 Deposition: 27/07/2020
Map released: 04/08/2021
Last modified: 10/07/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Nostoc punctiforme
Sample: vipp1 c17 ring
Fitted models: 6zw7 (Avg. Q-score: 0.211)

Deposition Authors: Liu J, Tassinari M, Souza DP , Naskar S , Noel JK, Bohuszewicz O, Buck M, Williams TA , Baum B , Low HH
Bacterial Vipp1 and PspA are members of the ancient ESCRT-III membrane-remodeling superfamily.
Liu J, Tassinari M, Souza DP , Naskar S , Noel JK, Bohuszewicz O, Buck M, Williams TA , Baum B , Low HH
(2021) Cell , 184 , 3660 - 3673.e18
PUBMED: 34166615
DOI: doi:10.1016/j.cell.2021.05.041
ISSN: 1097-4172
Abstract:
Membrane remodeling and repair are essential for all cells. Proteins that perform these functions include Vipp1/IM30 in photosynthetic plastids, PspA in bacteria, and ESCRT-III in eukaryotes. Here, using a combination of evolutionary and structural analyses, we show that these protein families are homologous and share a common ancient evolutionary origin that likely predates the last universal common ancestor. This homology is evident in cryo-electron microscopy structures of Vipp1 rings from the cyanobacterium Nostoc punctiforme presented over a range of symmetries. Each ring is assembled from rungs that stack and progressively tilt to form dome-shaped curvature. Assembly is facilitated by hinges in the Vipp1 monomer, similar to those in ESCRT-III proteins, which allow the formation of flexible polymers. Rings have an inner lumen that is able to bind and deform membranes. Collectively, these data suggest conserved mechanistic principles that underlie Vipp1, PspA, and ESCRT-III-dependent membrane remodeling across all domains of life.