EMD-11769

Single-particle
4.2 Å
EMD-11769 Deposition: 19/09/2020
Map released: 07/07/2021
Last modified: 24/04/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-11769

Bacterial 30S ribosomal subunit assembly complex state D (multibody refinement for body domain of 30S ribosome)

EMD-11769

Single-particle
4.2 Å
EMD-11769 Deposition: 19/09/2020
Map released: 07/07/2021
Last modified: 24/04/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Escherichia coli
Sample: Bacterial 30S ribosomal subunit assembly complex state D (body domain)
Fitted models: 7afl (Avg. Q-score: 0.347)

Deposition Authors: Schedlbauer A , Iturrioz I
A conserved rRNA switch is central to decoding site maturation on the small ribosomal subunit.
PUBMED: 34088665
DOI: doi:10.1126/sciadv.abf7547
ISSN: 2375-2548
Abstract:
While a structural description of the molecular mechanisms guiding ribosome assembly in eukaryotic systems is emerging, bacteria use an unrelated core set of assembly factors for which high-resolution structural information is still missing. To address this, we used single-particle cryo-electron microscopy to visualize the effects of bacterial ribosome assembly factors RimP, RbfA, RsmA, and RsgA on the conformational landscape of the 30S ribosomal subunit and obtained eight snapshots representing late steps in the folding of the decoding center. Analysis of these structures identifies a conserved secondary structure switch in the 16S ribosomal RNA central to decoding site maturation and suggests both a sequential order of action and molecular mechanisms for the assembly factors in coordinating and controlling this switch. Structural and mechanistic parallels between bacterial and eukaryotic systems indicate common folding features inherent to all ribosomes.