EMD-12977

Single-particle
3.0 Å
EMD-12977 Deposition: 19/05/2021
Map released: 26/01/2022
Last modified: 09/02/2022
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-12977

Thermophilic eukaryotic 80S ribosome at pe/E (TI)-POST state

EMD-12977

Single-particle
3.0 Å
EMD-12977 Deposition: 19/05/2021
Map released: 26/01/2022
Last modified: 09/02/2022
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Chaetomium thermophilum var. thermophilum DSM 1495
Sample: Chaetomium thermophilum 80S ribosome
Fitted models: 7old (Avg. Q-score: 0.458)

Deposition Authors: Kisonaite M , Wild K , Sinning I
High-resolution structures of a thermophilic eukaryotic 80S ribosome reveal atomistic details of translocation.
Kisonaite M , Wild K , Lapouge K , Ruppert T , Sinning I
(2022) Nat Commun , 13 , 476 - 476
PUBMED: 35079002
DOI: doi:10.1038/s41467-022-27967-9
ISSN: 2041-1723
Abstract:
Ribosomes are complex and highly conserved ribonucleoprotein assemblies catalyzing protein biosynthesis in every organism. Here we present high-resolution cryo-EM structures of the 80S ribosome from a thermophilic fungus in two rotational states, which due to increased 80S stability provide a number of mechanistic details of eukaryotic translation. We identify a universally conserved 'nested base-triple knot' in the 26S rRNA at the polypeptide tunnel exit with a bulged-out nucleotide that likely serves as an adaptable element for nascent chain containment and handover. We visualize the structure and dynamics of the ribosome protective factor Stm1 upon ribosomal 40S head swiveling. We describe the structural impact of a unique and essential m1acp3 Ψ 18S rRNA hyper-modification embracing the anticodon wobble-position for eukaryotic tRNA and mRNA translocation. We complete the eEF2-GTPase switch cycle describing the GDP-bound post-hydrolysis state. Taken together, our data and their integration into the structural landscape of 80S ribosomes furthers our understanding of protein biogenesis.