EMD-1314

Single-particle
24.0 Å
EMD-1314 Deposition: 02/01/2007
Map released: 11/06/2007
Last modified: 24/10/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-1314

Structure of immature West Nile virus.

EMD-1314

Single-particle
24.0 Å
EMD-1314 Deposition: 02/01/2007
Map released: 11/06/2007
Last modified: 24/10/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: West Nile virus
Sample: immature West Nile virus
Fitted models: 2of6 (Avg. Q-score: 0.052)

Deposition Authors: Zhang Y, Kaufmann B, Chipman PR , Kuhn RJ, Rossmann MG
Structure of immature West Nile virus.
Zhang Y, Kaufmann B, Chipman PR , Kuhn RJ, Rossmann MG
(2007) J.Virol. , 81 , 6141 - 6145
Abstract:
The structure of immature West Nile virus particles, propagated in the presence of ammonium chloride to block virus maturation in the low-pH environment of the trans-Golgi network, was determined by cryo-electron microscopy (cryo-EM). The structure of these particles was similar to that of immature West Nile virus particles found as a minor component of mature virus samples (naturally occurring immature particles [NOIPs]). The structures of mature infectious flaviviruses are radically different from those of the immature particles. The similarity of the ammonium chloride-treated particles and NOIPs suggests either that the NOIPs have not undergone any conformational change during maturation or that the conformational change is reversible. Comparison with the cryo-EM reconstruction of immature dengue virus established the locations of the N-linked glycosylation sites of these viruses, verifying the interpretation of the reconstructions of the immature flaviviruses.