EMD-1314
Structure of immature West Nile virus.
EMD-1314
Single-particle24.0 Å
Deposition: 02/01/2007
Map released: 11/06/2007
Last modified: 24/10/2012
Sample Organism:
West Nile virus
Sample: immature West Nile virus
Fitted models: 2of6 (Avg. Q-score: 0.052)
Deposition Authors: Zhang Y, Kaufmann B, Chipman PR , Kuhn RJ, Rossmann MG
Sample: immature West Nile virus
Fitted models: 2of6 (Avg. Q-score: 0.052)
Deposition Authors: Zhang Y, Kaufmann B, Chipman PR , Kuhn RJ, Rossmann MG
Structure of immature West Nile virus.
Abstract:
The structure of immature West Nile virus particles, propagated in the presence of ammonium chloride to block virus maturation in the low-pH environment of the trans-Golgi network, was determined by cryo-electron microscopy (cryo-EM). The structure of these particles was similar to that of immature West Nile virus particles found as a minor component of mature virus samples (naturally occurring immature particles [NOIPs]). The structures of mature infectious flaviviruses are radically different from those of the immature particles. The similarity of the ammonium chloride-treated particles and NOIPs suggests either that the NOIPs have not undergone any conformational change during maturation or that the conformational change is reversible. Comparison with the cryo-EM reconstruction of immature dengue virus established the locations of the N-linked glycosylation sites of these viruses, verifying the interpretation of the reconstructions of the immature flaviviruses.
The structure of immature West Nile virus particles, propagated in the presence of ammonium chloride to block virus maturation in the low-pH environment of the trans-Golgi network, was determined by cryo-electron microscopy (cryo-EM). The structure of these particles was similar to that of immature West Nile virus particles found as a minor component of mature virus samples (naturally occurring immature particles [NOIPs]). The structures of mature infectious flaviviruses are radically different from those of the immature particles. The similarity of the ammonium chloride-treated particles and NOIPs suggests either that the NOIPs have not undergone any conformational change during maturation or that the conformational change is reversible. Comparison with the cryo-EM reconstruction of immature dengue virus established the locations of the N-linked glycosylation sites of these viruses, verifying the interpretation of the reconstructions of the immature flaviviruses.