EMD-13603
Asymmetric single particle reconstruction of the Haliangium ochraceum encapsulin encapsulated ferritin complex calculated using Cryosparc
EMD-13603
Single-particle3.24 Å
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Map released: 09/02/2022
Last modified: 09/02/2022
Sample Organism:
Haliangium ochraceum
Sample: Ternary complex of Haliangium ochraceum encapsulin and encapsulated ferritin proteins
Deposition Authors: Marles-Wright J
,
Basle A
,
Ross J
,
Clarke DJ
Sample: Ternary complex of Haliangium ochraceum encapsulin and encapsulated ferritin proteins
Deposition Authors: Marles-Wright J
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Pore dynamics and asymmetric cargo loading in an encapsulin nanocompartment.
Ross J
,
McIver Z
,
Lambert T
,
Piergentili C,
Bird JE
,
Gallagher KJ
,
Cruickshank FL
,
James P
,
Zarazua-Arvizu E
,
Horsfall LE
,
Waldron KJ
,
Wilson MD
,
Mackay CL,
Basle A
,
Clarke DJ
,
Marles-Wright J
(2022) Sci Adv , 8 , eabj4461 - eabj4461
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(2022) Sci Adv , 8 , eabj4461 - eabj4461
Abstract:
Encapsulins are protein nanocompartments that house various cargo enzymes, including a family of decameric ferritin-like proteins. Here, we study a recombinant Haliangium ochraceum encapsulin:encapsulated ferritin complex using cryo-electron microscopy and hydrogen/deuterium exchange mass spectrometry to gain insight into the structural relationship between the encapsulin shell and its protein cargo. An asymmetric single-particle reconstruction reveals four encapsulated ferritin decamers in a tetrahedral arrangement within the encapsulin nanocompartment. This leads to a symmetry mismatch between the protein cargo and the icosahedral encapsulin shell. The encapsulated ferritin decamers are offset from the interior face of the encapsulin shell. Using hydrogen/deuterium exchange mass spectrometry, we observed the dynamic behavior of the major fivefold pore in the encapsulin shell and show the pore opening via the movement of the encapsulin A-domain. These data will accelerate efforts to engineer the encapsulation of heterologous cargo proteins and to alter the permeability of the encapsulin shell via pore modifications.
Encapsulins are protein nanocompartments that house various cargo enzymes, including a family of decameric ferritin-like proteins. Here, we study a recombinant Haliangium ochraceum encapsulin:encapsulated ferritin complex using cryo-electron microscopy and hydrogen/deuterium exchange mass spectrometry to gain insight into the structural relationship between the encapsulin shell and its protein cargo. An asymmetric single-particle reconstruction reveals four encapsulated ferritin decamers in a tetrahedral arrangement within the encapsulin nanocompartment. This leads to a symmetry mismatch between the protein cargo and the icosahedral encapsulin shell. The encapsulated ferritin decamers are offset from the interior face of the encapsulin shell. Using hydrogen/deuterium exchange mass spectrometry, we observed the dynamic behavior of the major fivefold pore in the encapsulin shell and show the pore opening via the movement of the encapsulin A-domain. These data will accelerate efforts to engineer the encapsulation of heterologous cargo proteins and to alter the permeability of the encapsulin shell via pore modifications.