Abstract:
Members of the ubiquitous SLC11/NRAMP family catalyze the uptake of divalent transition metal ions into cells. They have evolved to efficiently select these trace elements from a large pool of Ca²⁺ and Mg²⁺, which are both orders of magnitude more abundant, and to concentrate them in the cytoplasm aided by the cotransport of H⁺ serving as energy source. In the present study, we have characterized a member of a distant clade of the family found in prokaryotes, termed NRMTs, that were proposed to function as transporters of Mg²⁺. The protein transports Mg²⁺ and Mn²⁺ but not Ca²⁺ by a mechanism that is not coupled to H⁺. Structures determined by cryo-EM and X-ray crystallography revealed a generally similar protein architecture compared to classical NRAMPs, with a restructured ion binding site whose increased volume provides suitable interactions with ions that likely have retained much of their hydration shell.