EMD-1475

Single-particle
29.0 Å
EMD-1475 Deposition: 22/02/2008
Map released: 31/03/2009
Last modified: 05/05/2009
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-1475

Structure of bacteriophage N4

EMD-1475

Single-particle
29.0 Å
EMD-1475 Deposition: 22/02/2008
Map released: 31/03/2009
Last modified: 05/05/2009
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Enterobacteria phage N4
Sample: Bacteriophage N4

Deposition Authors: Choi KH, McPartland J, Kaganman I, Bowman VD, Rothman-Denes LB, Rossmann MG
Insight into DNA and protein transport in double-stranded DNA viruses: the structure of bacteriophage N4.
Choi KH, McPartland J, Kaganman I, Bowman VD, Rothman-Denes LB, Rossmann MG
(2008) J. Mol. Biol. , 378 , 726 - 736
Abstract:
Bacteriophage N4 encapsidates a 3500-aa-long DNA-dependent RNA polymerase (vRNAP), which is injected into the host along with the N4 genome upon infection. The three-dimensional structures of wild-type and mutant N4 viruses lacking gp17, gp50, or gp65 were determined by cryoelectron microscopy. The virion has an icosahedral capsid with T=9 quasi-symmetry that encapsidates well-organized double-stranded DNA and vRNAP. The tail, attached at a unique pentameric vertex of the head, consists of a neck, 12 appendages, and six ribbons that constitute a non-contractile sheath around a central tail tube. Comparison of wild-type and mutant virus structures in conjunction with bioinformatics established the identity and virion locations of the major capsid protein (gp56), a decorating protein (gp17), the vRNAP (gp50), the tail sheath (gp65), the appendages (gp66), and the portal protein (gp59). The N4 virion organization provides insight into its assembly and suggests a mechanism for genome and vRNAP transport strategies utilized by this unique system.