EMD-15674

Single-particle
2.38 Å
EMD-15674 Deposition: 25/08/2022
Map released: 07/06/2023
Last modified: 24/04/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-15674

Cryo-EM structure of the plant 40S subunit

EMD-15674

Single-particle
2.38 Å
EMD-15674 Deposition: 25/08/2022
Map released: 07/06/2023
Last modified: 24/04/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Nicotiana tabacum
Sample: 40S subunit of the cytosolic 80S ribosome from tobacco
Fitted models: 8auv (Avg. Q-score: 0.622)

Deposition Authors: Smirnova J , Loerke J , Kleinau G , Schmidt A , Buerger J, Meyer EH , Mielke T , Scheerer P , Bock R , Spahn CMT , Zoschke R
Structure of the actively translating plant 80S ribosome at 2.2 angstrom resolution.
Smirnova J , Loerke J , Kleinau G , Schmidt A , Burger J, Meyer EH , Mielke T , Scheerer P , Bock R , Spahn CMT , Zoschke R
(2023) Nat Plants , 9 , 987 - 1000
PUBMED: 37156858
DOI: doi:10.1038/s41477-023-01407-y
ISSN: 2055-0278
Abstract:
In plant cells, translation occurs in three compartments: the cytosol, the plastids and the mitochondria. While the structures of the (prokaryotic-type) ribosomes in plastids and mitochondria are well characterized, high-resolution structures of the eukaryotic 80S ribosomes in the cytosol have been lacking. Here the structure of translating tobacco (Nicotiana tabacum) 80S ribosomes was solved by cryo-electron microscopy with a global resolution of 2.2 Å. The ribosome structure includes two tRNAs, decoded mRNA and the nascent peptide chain, thus providing insights into the molecular underpinnings of the cytosolic translation process in plants. The map displays conserved and plant-specific rRNA modifications and the positions of numerous ionic cofactors, and it uncovers the role of monovalent ions in the decoding centre. The model of the plant 80S ribosome enables broad phylogenetic comparisons that reveal commonalities and differences in the ribosomes of plants and those of other eukaryotes, thus putting our knowledge about eukaryotic translation on a firmer footing.