EMD-16725

Subtomogram averaging
3.4 Å
EMD-16725 Deposition: 19/02/2023
Map released: 19/07/2023
Last modified: 19/07/2023
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-16725

Human 80S ribosome at the 'eEF1A, A/T, P' state in cells

EMD-16725

Subtomogram averaging
3.4 Å
EMD-16725 Deposition: 19/02/2023
Map released: 19/07/2023
Last modified: 19/07/2023
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Homo sapiens
Sample: Human 80S ribosome at the 'eEF1A, A/T, P' state in cells

Deposition Authors: Xing H , Welsch S , Turonova B , Beck M
Translation dynamics in human cells visualized at high resolution reveal cancer drug action.
Xing H , Taniguchi R , Khusainov I , Kreysing JP , Welsch S , Turonova B , Beck M
(2023) Science , 381 , 70 - 75
PUBMED: 37410833
DOI: doi:10.1126/science.adh1411
ISSN: 1095-9203
ASTM: SCIEAS
Abstract:
Ribosomes catalyze protein synthesis by cycling through various functional states. These states have been extensively characterized in vitro, but their distribution in actively translating human cells remains elusive. We used a cryo-electron tomography-based approach and resolved ribosome structures inside human cells with high resolution. These structures revealed the distribution of functional states of the elongation cycle, a Z transfer RNA binding site, and the dynamics of ribosome expansion segments. Ribosome structures from cells treated with Homoharringtonine, a drug used against chronic myeloid leukemia, revealed how translation dynamics were altered in situ and resolve the small molecules within the active site of the ribosome. Thus, structural dynamics and drug effects can be assessed at high resolution within human cells.