EMD-16725
Human 80S ribosome at the 'eEF1A, A/T, P' state in cells
EMD-16725
Subtomogram averaging3.4 Å
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Map released: 19/07/2023
Last modified: 19/07/2023
Sample Organism:
Homo sapiens
Sample: Human 80S ribosome at the 'eEF1A, A/T, P' state in cells
Deposition Authors: Xing H
,
Welsch S
,
Turonova B
,
Beck M
Sample: Human 80S ribosome at the 'eEF1A, A/T, P' state in cells
Deposition Authors: Xing H
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Translation dynamics in human cells visualized at high resolution reveal cancer drug action.
Xing H
,
Taniguchi R
,
Khusainov I
,
Kreysing JP
,
Welsch S
,
Turonova B
,
Beck M
(2023) Science , 381 , 70 - 75
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(2023) Science , 381 , 70 - 75
Abstract:
Ribosomes catalyze protein synthesis by cycling through various functional states. These states have been extensively characterized in vitro, but their distribution in actively translating human cells remains elusive. We used a cryo-electron tomography-based approach and resolved ribosome structures inside human cells with high resolution. These structures revealed the distribution of functional states of the elongation cycle, a Z transfer RNA binding site, and the dynamics of ribosome expansion segments. Ribosome structures from cells treated with Homoharringtonine, a drug used against chronic myeloid leukemia, revealed how translation dynamics were altered in situ and resolve the small molecules within the active site of the ribosome. Thus, structural dynamics and drug effects can be assessed at high resolution within human cells.
Ribosomes catalyze protein synthesis by cycling through various functional states. These states have been extensively characterized in vitro, but their distribution in actively translating human cells remains elusive. We used a cryo-electron tomography-based approach and resolved ribosome structures inside human cells with high resolution. These structures revealed the distribution of functional states of the elongation cycle, a Z transfer RNA binding site, and the dynamics of ribosome expansion segments. Ribosome structures from cells treated with Homoharringtonine, a drug used against chronic myeloid leukemia, revealed how translation dynamics were altered in situ and resolve the small molecules within the active site of the ribosome. Thus, structural dynamics and drug effects can be assessed at high resolution within human cells.