EMD-17719

Single-particle
3.03 Å
EMD-17719 Deposition: 23/06/2023
Map released: 13/12/2023
Last modified: 26/06/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-17719

human mitoribosomal large subunit assembly intermediate 1 with GTPBP10-GTPBP7

EMD-17719

Single-particle
3.03 Å
EMD-17719 Deposition: 23/06/2023
Map released: 13/12/2023
Last modified: 26/06/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Homo sapiens
Sample: large mitoribosomal subunit biogenesis intermediate with GTPBP10, GTPBP7, NSUN4-MTERF4, ACP-module
Fitted models: 8pk0 (Avg. Q-score: 0.455)

Deposition Authors: Kummer E , Nguyen TG, Ritter C
Structural insights into the role of GTPBP10 in the RNA maturation of the mitoribosome.
Nguyen TG, Ritter C , Kummer E
(2023) Nat Commun , 14 , 7991 - 7991
PUBMED: 38042949
DOI: doi:10.1038/s41467-023-43599-z
ISSN: 2041-1723
Abstract:
Mitochondria contain their own genetic information and a dedicated translation system to express it. The mitochondrial ribosome is assembled from mitochondrial-encoded RNA and nuclear-encoded ribosomal proteins. Assembly is coordinated in the mitochondrial matrix by biogenesis factors that transiently associate with the maturing particle. Here, we present a structural snapshot of a large mitoribosomal subunit assembly intermediate containing 7 biogenesis factors including the GTPases GTPBP7 and GTPBP10. Our structure illustrates how GTPBP10 aids the folding of the ribosomal RNA during the biogenesis process, how this process is related to bacterial ribosome biogenesis, and why mitochondria require two biogenesis factors in contrast to only one in bacteria.