EMD-17848

Single-particle
3.6 Å
EMD-17848 Deposition: 13/07/2023
Map released: 22/11/2023
Last modified: 23/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-17848

Asymmetric unit of the yeast fatty acid synthase in the semi non-rotated state with ACP at the ketosynthase domain (FASam sample)

EMD-17848

Single-particle
3.6 Å
EMD-17848 Deposition: 13/07/2023
Map released: 22/11/2023
Last modified: 23/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Saccharomyces cerevisiae
Sample: Yeast fatty acid synthase
Fitted models: 8psa (Avg. Q-score: 0.423)

Deposition Authors: Singh K , Bunzel G, Graf B, Yip KM, Stark H, Chari A
Reconstruction of a fatty acid synthesis cycle from acyl carrier protein and cofactor structural snapshots.
Singh K , Bunzel G, Graf B, Yip KM, Neumann-Schaal M, Stark H, Chari A
(2023) Cell , 186 , 5054 - 5067.e16
PUBMED: 37949058
DOI: doi:10.1016/j.cell.2023.10.009
ISSN: 1097-4172
Abstract:
Fatty acids (FAs) play a central metabolic role in living cells as constituents of membranes, cellular energy reserves, and second messenger precursors. A 2.6 MDa FA synthase (FAS), where the enzymatic reactions and structures are known, is responsible for FA biosynthesis in yeast. Essential in the yeast FAS catalytic cycle is the acyl carrier protein (ACP) that actively shuttles substrates, biosynthetic intermediates, and products from one active site to another. We resolve the S. cerevisiae FAS structure at 1.9 Å, elucidating cofactors and water networks involved in their recognition. Structural snapshots of ACP domains bound to various enzymatic domains allow the reconstruction of a full yeast FA biosynthesis cycle. The structural information suggests that each FAS functional unit could accommodate exogenous proteins to incorporate various enzymatic activities, and we show proof-of-concept experiments where ectopic proteins are used to modulate FAS product profiles.