EMD-17945

Single-particle
8.64 Å
EMD-17945 Deposition: 17/07/2023
Map released: 17/07/2024
Last modified: 17/07/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-17945

Tick-borne encephalitis virus (strain HYPR) immature particle

EMD-17945

Single-particle
8.64 Å
EMD-17945 Deposition: 17/07/2023
Map released: 17/07/2024
Last modified: 17/07/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Tick-borne encephalitis virus
Sample: Tick-borne encephalitis virus

Deposition Authors: Fuzik T , Plevka P , Smerdova L , Novacek J
The structure of immature tick-borne encephalitis virus supports the collapse model of flavivirus maturation.
Anastasina M , Fuzik T , Domanska A , Pulkkinen LIA , Smerdova L , Formanova PP , Strakova P , Novacek J , Ruzek D , Plevka P , Butcher SJ
(2024) Sci Adv , 10 , eadl1888 - eadl1888
PUBMED: 38959313
DOI: doi:10.1126/sciadv.adl1888
ISSN: 2375-2548
Abstract:
We present structures of three immature tick-borne encephalitis virus (TBEV) isolates. Our atomic models of the major viral components, the E and prM proteins, indicate that the pr domains of prM have a critical role in holding the heterohexameric prM3E3 spikes in a metastable conformation. Destabilization of the prM furin-sensitive loop at acidic pH facilitates its processing. The prM topology and domain assignment in TBEV is similar to the mosquito-borne Binjari virus, but is in contrast to other immature flavivirus models. These results support that prM cleavage, the collapse of E protein ectodomains onto the virion surface, the large movement of the membrane domains of both E and M, and the release of the pr fragment from the particle render the virus mature and infectious. Our work favors the collapse model of flavivirus maturation warranting further studies of immature flaviviruses to determine the sequence of events and mechanistic details driving flavivirus maturation.