EMD-19555

Tomography
EMD-19555 Deposition: 05/02/2024
Map released: 26/06/2024
Last modified: 03/07/2024
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EMD-19555

cryo-tomogram of FIB-milled meiotic yeast cell containing filaments in the cytoplasm

EMD-19555

Tomography
EMD-19555 Deposition: 05/02/2024
Map released: 26/06/2024
Last modified: 03/07/2024
Overview Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Saccharomyces cerevisiae
Sample: cryo FIB-milled meiotic yeast cells

Deposition Authors: Hugener J , Xu J , Wettstein R, Ioannidi L, Velikov D , Wollweber F, Henggeler A , Matos J , Pilhofer M
FilamentID reveals the composition and function of metabolic enzyme polymers during gametogenesis.
Hugener J , Xu J , Wettstein R, Ioannidi L, Velikov D , Wollweber F, Henggeler A , Matos J , Pilhofer M
(2024) Cell , 187 , 3303 - 3318.e18
PUBMED: 38906101
DOI: doi:10.1016/j.cell.2024.04.026
ISSN: 1097-4172
Abstract:
Gamete formation and subsequent offspring development often involve extended phases of suspended cellular development or even dormancy. How cells adapt to recover and resume growth remains poorly understood. Here, we visualized budding yeast cells undergoing meiosis by cryo-electron tomography (cryoET) and discovered elaborate filamentous assemblies decorating the nucleus, cytoplasm, and mitochondria. To determine filament composition, we developed a "filament identification" (FilamentID) workflow that combines multiscale cryoET/cryo-electron microscopy (cryoEM) analyses of partially lysed cells or organelles. FilamentID identified the mitochondrial filaments as being composed of the conserved aldehyde dehydrogenase Ald4ALDH2 and the nucleoplasmic/cytoplasmic filaments as consisting of acetyl-coenzyme A (CoA) synthetase Acs1ACSS2. Structural characterization further revealed the mechanism underlying polymerization and enabled us to genetically perturb filament formation. Acs1 polymerization facilitates the recovery of chronologically aged spores and, more generally, the cell cycle re-entry of starved cells. FilamentID is broadly applicable to characterize filaments of unknown identity in diverse cellular contexts.