EMD-19676

Single-particle
2.97 Å
EMD-19676 Deposition: 17/02/2024
Map released: 25/12/2024
Last modified: 25/12/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-19676

CRYO-EM FOCUSED REFINEMENT MAP OF LEISHMANIA MAJOR 80S RIBOSOME WITH A/P/E-site tRNA AND mRNA : LM32Cs3H1 sKO STRAIN

EMD-19676

Single-particle
2.97 Å
EMD-19676 Deposition: 17/02/2024
Map released: 25/12/2024
Last modified: 25/12/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Leishmania major strain Friedlin
Sample: 80S RIBOSOME WITH A/P/E-site tRNA AND mRNA

Deposition Authors: Rajan KS , Yonath A
Structural and mechanistic insights into the function of Leishmania ribosome lacking a single pseudouridine modification.
PUBMED: 38722744
DOI: doi:10.1016/j.celrep.2024.114203
ISSN: 2211-1247
Abstract:
Leishmania is the causative agent of cutaneous and visceral diseases affecting millions of individuals worldwide. Pseudouridine (Ψ), the most abundant modification on rRNA, changes during the parasite life cycle. Alterations in the level of a specific Ψ in helix 69 (H69) affected ribosome function. To decipher the molecular mechanism of this phenotype, we determine the structure of ribosomes lacking the single Ψ and its parental strain at ∼2.4-3 Å resolution using cryo-EM. Our findings demonstrate the significance of a single Ψ on H69 to its structure and the importance for its interactions with helix 44 and specific tRNAs. Our study suggests that rRNA modification affects translation of mRNAs carrying codon bias due to selective accommodation of tRNAs by the ribosome. Based on the high-resolution structures, we propose a mechanism explaining how the ribosome selects specific tRNAs.