EMD-19676
CRYO-EM FOCUSED REFINEMENT MAP OF LEISHMANIA MAJOR 80S RIBOSOME WITH A/P/E-site tRNA AND mRNA : LM32Cs3H1 sKO STRAIN
EMD-19676
Single-particle2.97 Å
![EMD-19676](https://www.ebi.ac.uk/emdb/images/entry/EMD-19676/400_19676.gif)
Map released: 25/12/2024
Last modified: 25/12/2024
Sample Organism:
Leishmania major strain Friedlin
Sample: 80S RIBOSOME WITH A/P/E-site tRNA AND mRNA
Deposition Authors: Rajan KS
,
Yonath A
Sample: 80S RIBOSOME WITH A/P/E-site tRNA AND mRNA
Deposition Authors: Rajan KS
![](http://www.ebi.ac.uk/web_guidelines/images/logos/orcid/orcid_16x16.png)
Structural and mechanistic insights into the function of Leishmania ribosome lacking a single pseudouridine modification.
Rajan KS
,
Aryal S,
Hiregange DG,
Bashan A,
Madmoni H,
Olami M
,
Doniger T,
Cohen-Chalamish S,
Pescher P,
Taoka M
,
Nobe Y,
Fedorenko A,
Bose T,
Zimermann E,
Prina E,
Aharon-Hefetz N,
Pilpel Y,
Isobe T,
Unger R,
Spath GF,
Yonath A,
Michaeli S
(2024) Cell Rep , 43 , 114203 - 114203
![](http://www.ebi.ac.uk/web_guidelines/images/logos/orcid/orcid_16x16.png)
![](http://www.ebi.ac.uk/web_guidelines/images/logos/orcid/orcid_16x16.png)
![](http://www.ebi.ac.uk/web_guidelines/images/logos/orcid/orcid_16x16.png)
(2024) Cell Rep , 43 , 114203 - 114203
Abstract:
Leishmania is the causative agent of cutaneous and visceral diseases affecting millions of individuals worldwide. Pseudouridine (Ψ), the most abundant modification on rRNA, changes during the parasite life cycle. Alterations in the level of a specific Ψ in helix 69 (H69) affected ribosome function. To decipher the molecular mechanism of this phenotype, we determine the structure of ribosomes lacking the single Ψ and its parental strain at ∼2.4-3 Å resolution using cryo-EM. Our findings demonstrate the significance of a single Ψ on H69 to its structure and the importance for its interactions with helix 44 and specific tRNAs. Our study suggests that rRNA modification affects translation of mRNAs carrying codon bias due to selective accommodation of tRNAs by the ribosome. Based on the high-resolution structures, we propose a mechanism explaining how the ribosome selects specific tRNAs.
Leishmania is the causative agent of cutaneous and visceral diseases affecting millions of individuals worldwide. Pseudouridine (Ψ), the most abundant modification on rRNA, changes during the parasite life cycle. Alterations in the level of a specific Ψ in helix 69 (H69) affected ribosome function. To decipher the molecular mechanism of this phenotype, we determine the structure of ribosomes lacking the single Ψ and its parental strain at ∼2.4-3 Å resolution using cryo-EM. Our findings demonstrate the significance of a single Ψ on H69 to its structure and the importance for its interactions with helix 44 and specific tRNAs. Our study suggests that rRNA modification affects translation of mRNAs carrying codon bias due to selective accommodation of tRNAs by the ribosome. Based on the high-resolution structures, we propose a mechanism explaining how the ribosome selects specific tRNAs.