EMD-21274
Polyclonal Fabs from rhesus macaque rh2011 wk28 plasma in complex with HIV Env trimer BG505 SOSIP.v5.2
EMD-21274
Single-particle19.0 Å
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Map released: 01/07/2020
Last modified: 16/09/2020
Sample Organism:
Macaca mulatta
Sample: Polyclonal Fabs from rhesus macaque rh2011 wk28 plasma in complex with HIV Env trimer BG505 SOSIP.v5.2
Deposition Authors: Cottrell CA, Sewall LM, Ward AB
Sample: Polyclonal Fabs from rhesus macaque rh2011 wk28 plasma in complex with HIV Env trimer BG505 SOSIP.v5.2
Deposition Authors: Cottrell CA, Sewall LM, Ward AB
Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates.
Cottrell CA
,
van Schooten J
,
Bowman CA
,
Yuan M
,
Oyen D,
Shin M
,
Morpurgo R
,
van der Woude P,
van Breemen M,
Torres JL
,
Patel R,
Gross J
,
Sewall LM
,
Copps J
,
Ozorowski G
,
Nogal B
,
Sok D
,
Rakasz EG,
Labranche C
,
Vigdorovich V
,
Christley S,
Carnathan DG,
Sather DN
,
Montefiori D
,
Silvestri G
,
Burton DR
,
Moore JP,
Wilson IA,
Sanders RW
,
Ward AB
,
van Gils MJ
(2020) Plos Pathog. , 16 , e1008753 - e1008753
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(2020) Plos Pathog. , 16 , e1008753 - e1008753
Abstract:
The induction of broad and potent immunity by vaccines is the key focus of research efforts aimed at protecting against HIV-1 infection. Soluble native-like HIV-1 envelope glycoproteins have shown promise as vaccine candidates as they can induce potent autologous neutralizing responses in rabbits and non-human primates. In this study, monoclonal antibodies were isolated and characterized from rhesus macaques immunized with the BG505 SOSIP.664 trimer to better understand vaccine-induced antibody responses. Our studies reveal a diverse landscape of antibodies recognizing immunodominant strain-specific epitopes and non-neutralizing neo-epitopes. Additionally, we isolated a subset of mAbs against an epitope cluster at the gp120-gp41 interface that recognize the highly conserved fusion peptide and the glycan at position 88 and have characteristics akin to several human-derived broadly neutralizing antibodies.
The induction of broad and potent immunity by vaccines is the key focus of research efforts aimed at protecting against HIV-1 infection. Soluble native-like HIV-1 envelope glycoproteins have shown promise as vaccine candidates as they can induce potent autologous neutralizing responses in rabbits and non-human primates. In this study, monoclonal antibodies were isolated and characterized from rhesus macaques immunized with the BG505 SOSIP.664 trimer to better understand vaccine-induced antibody responses. Our studies reveal a diverse landscape of antibodies recognizing immunodominant strain-specific epitopes and non-neutralizing neo-epitopes. Additionally, we isolated a subset of mAbs against an epitope cluster at the gp120-gp41 interface that recognize the highly conserved fusion peptide and the glycan at position 88 and have characteristics akin to several human-derived broadly neutralizing antibodies.