EMD-21491

Single-particle
3.0 Å
EMD-21491 Deposition: 28/02/2020
Map released: 11/03/2020
Last modified: 22/07/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-21491

Thermoplasma acidophilum 20S proteasome cryo-EM structure using automatic preprocessing workflow

EMD-21491

Single-particle
3.0 Å
EMD-21491 Deposition: 28/02/2020
Map released: 11/03/2020
Last modified: 22/07/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Thermoplasma acidophilum
Sample: T20S Proteasome

Deposition Authors: Cianfrocco MA, Li Y
High-Throughput Cryo-EM Enabled by User-Free Preprocessing Routines.
Li Y, Cash JN, Tesmer JJG, Cianfrocco MA
(2020) Structure , 28 , 858 - 869.e3
PUBMED: 32294468
DOI: doi:10.1016/j.str.2020.03.008
ISSN: 0969-2126
ASTM: STRUE6
Abstract:
Single-particle cryoelectron microscopy (cryo-EM) continues to grow into a mainstream structural biology technique. Recent developments in data collection strategies alongside new sample preparation devices herald a future where users will collect multiple datasets per microscope session. To make cryo-EM data processing more automatic and user-friendly, we have developed an automatic pipeline for cryo-EM data preprocessing and assessment using a combination of deep-learning and image-analysis tools. We have verified the performance of this pipeline on a number of datasets and extended its scope to include sample screening by the user-free assessment of the qualities of a series of datasets under different conditions. We propose that our workflow provides a decision-free solution for cryo-EM, making data preprocessing more generalized and robust in the high-throughput era as well as more convenient for users from a range of backgrounds.