EMD-2176

Single-particle
25.0 Å
EMD-2176 Deposition: 11/08/2012
Map released: 03/10/2012
Last modified: 03/10/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2176

Negative stain electron microscopy of a CSN complex

EMD-2176

Single-particle
25.0 Å
EMD-2176 Deposition: 11/08/2012
Map released: 03/10/2012
Last modified: 03/10/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Homo sapiens
Sample: CSN

Deposition Authors: Enchev RI, Scott DC, da Fonseca P, Schreiber A , Monda JK, Schulman BA , Peter M , Morris EP
Structural basis for a reciprocal regulation between SCF and CSN.
Enchev RI, Scott DC, da Fonseca PC , Schreiber A , Monda JK, Schulman BA , Peter M , Morris EP
(2012) Cell Rep , 2 , 616 - 627
Abstract:
Skp1-Cul1-Fbox (SCF) E3 ligases are activated by ligation to the ubiquitin-like protein Nedd8, which is reversed by the deneddylating Cop9 signalosome (CSN). However, CSN also promotes SCF substrate turnover through unknown mechanisms. Through biochemical and electron microscopy analyses, we determined molecular models of CSN complexes with SCF(Skp2/Cks1) and SCF(Fbw7) and found that CSN occludes both SCF functional sites-the catalytic Rbx1-Cul1 C-terminal domain and the substrate receptor. Indeed, CSN binding prevents SCF interactions with E2 enzymes and a ubiquitination substrate, and it inhibits SCF-catalyzed ubiquitin chain formation independent of deneddylation. Importantly, CSN prevents neddylation of the bound cullin, unless binding of a ubiquitination substrate triggers SCF dissociation and neddylation. Taken together, the results provide a model for how reciprocal regulation sensitizes CSN to the SCF assembly state and inhibits a catalytically competent SCF until a ubiquitination substrate drives its own degradation by displacing CSN, thereby promoting cullin neddylation and substrate ubiquitination.