EMD-2367

Single-particle
13.0 Å
EMD-2367 Deposition: 22/04/2013
Map released: 19/06/2013
Last modified: 17/07/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2367

Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5

EMD-2367

Single-particle
13.0 Å
EMD-2367 Deposition: 22/04/2013
Map released: 19/06/2013
Last modified: 17/07/2013
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: unidentified, Homo sapiens
Sample: Human Pol II in complex with artificial transcription bubble and RECQL5

Deposition Authors: Kassube SA , Jinek M , Fang J, Tsutakawa S , Nogales E
Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5.
Kassube SA , Jinek M , Fang J, Tsutakawa S , Nogales E
(2013) Nat. Struct. Mol. Biol. , 20 , 892 - 899
Abstract:
RECQL5 is a member of the highly conserved RecQ family of DNA helicases involved in DNA repair. RECQL5 interacts with RNA polymerase II (Pol II) and inhibits transcription of protein-encoding genes by an unknown mechanism. We show that RECQL5 contacts the Rpb1 jaw domain of Pol II at a site that overlaps with the binding site for the transcription elongation factor TFIIS. Our cryo-EM structure of elongating Pol II arrested in complex with RECQL5 shows that the RECQL5 helicase domain is positioned to sterically block elongation. The crystal structure of the RECQL5 KIX domain reveals similarities with TFIIS, and binding of RECQL5 to Pol II interferes with the ability of TFIIS to promote transcriptional read-through in vitro. Together, our findings reveal a dual mode of transcriptional repression by RECQL5 that includes structural mimicry of the Pol II-TFIIS interaction.