EMD-2394
Architecture of an RNA polymerase II transcription pre-initiation complex
EMD-2394
Single-particle16.0 Å
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Map released: 02/10/2013
Last modified: 20/11/2013
Sample Organism:
Saccharomyces cerevisiae
Sample: RNA polymerase II transcription pre-initiation complex
Deposition Authors: Murakami K
,
Elmlund H,
Kalisman N
,
Bushnell DA,
Adams CM,
Azubel M,
Elmlund D,
Levi-Kalisman Y,
Liu X
,
Levitt M,
Kornberg RD
Sample: RNA polymerase II transcription pre-initiation complex
Deposition Authors: Murakami K
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Architecture of an RNA Polymerase II Transcription Pre-Initiation Complex.
Murakami K
,
Elmlund H,
Kalisman N
,
Bushnell DA,
Adams CM,
Azubel M,
Elmlund D,
Levi-Kalisman Y,
Liu X
,
Levitt M,
Kornberg RD
(2013) Science , 342 , 1238724 - 1238724
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(2013) Science , 342 , 1238724 - 1238724
Abstract:
The protein density and arrangement of subunits of a complete, 32-protein, RNA polymerase II (pol II) transcription pre-initiation complex (PIC) were determined by means of cryogenic electron microscopy and a combination of chemical cross-linking and mass spectrometry. The PIC showed a marked division in two parts, one containing all the general transcription factors (GTFs) and the other pol II. Promoter DNA was associated only with the GTFs, suspended above the pol II cleft and not in contact with pol II. This structural principle of the PIC underlies its conversion to a transcriptionally active state; the PIC is poised for the formation of a transcription bubble and descent of the DNA into the pol II cleft.
The protein density and arrangement of subunits of a complete, 32-protein, RNA polymerase II (pol II) transcription pre-initiation complex (PIC) were determined by means of cryogenic electron microscopy and a combination of chemical cross-linking and mass spectrometry. The PIC showed a marked division in two parts, one containing all the general transcription factors (GTFs) and the other pol II. Promoter DNA was associated only with the GTFs, suspended above the pol II cleft and not in contact with pol II. This structural principle of the PIC underlies its conversion to a transcriptionally active state; the PIC is poised for the formation of a transcription bubble and descent of the DNA into the pol II cleft.