EMD-2420
Electron microscopy of the CSM/RNA complex from Sulfolobus solfataricus
EMD-2420
Single-particle30.0 Å
Deposition: 19/07/2013Map released: 23/04/2014
Last modified: 21/05/2014
Sample Organism:
Sulfolobus solfataricus
Sample: CSM/RNA complex from Sulfolobus solfataricus
Deposition Authors: Rouillon C
,
Zhou M,
Zhang J,
Politis A,
Beilsten V ,
Cannone G,
Graham S ,
Robinson CV ,
Spagnolo L ,
White MF
Sample: CSM/RNA complex from Sulfolobus solfataricus
Deposition Authors: Rouillon C
,
Zhou M,
Zhang J,
Politis A,
Beilsten V ,
Cannone G,
Graham S ,
Robinson CV ,
Spagnolo L ,
White MF
Structure of the CRISPR Surveillance Complex CSM reveals key similarities with Cascade
Rouillon C ,
Zhou M,
Zhang J,
Politis A,
Beilsten V ,
Cannone G,
Graham S ,
Robinson CV ,
Spagnolo L ,
White MF
(2013) Mol. Cell , 52 , 124 - 134
,
Zhou M,
Zhang J,
Politis A,
Beilsten V ,
Cannone G,
Graham S ,
Robinson CV ,
Spagnolo L ,
White MF
(2013) Mol. Cell , 52 , 124 - 134
Abstract:
The Clustered Regularly Interspaced Palindromic Repeats (CRISPR) system is an adaptive immune system in prokaryotes. Interference complexes encoded by CRISPR-associated (cas) genes utilize small RNAs for homology-directed detection and subsequent degradation of invading genetic elements, and they have been classified into three main types (I-III). Type III complexes share the Cas10 subunit but are subclassifed as type IIIA (CSM) and type IIIB (CMR), depending on their specificity for DNA or RNA targets, respectively. The role of CSM in limiting the spread of conjugative plasmids in Staphylococcus epidermidis was first described in 2008. Here, we report a detailed investigation of the composition and structure of the CSM complex from the archaeon Sulfolobus solfataricus, using a combination of electron microscopy, mass spectrometry, and deep sequencing. This reveals a three-dimensional model for the CSM complex that includes a helical component strikingly reminiscent of the backbone structure of the type I (Cascade) family.
The Clustered Regularly Interspaced Palindromic Repeats (CRISPR) system is an adaptive immune system in prokaryotes. Interference complexes encoded by CRISPR-associated (cas) genes utilize small RNAs for homology-directed detection and subsequent degradation of invading genetic elements, and they have been classified into three main types (I-III). Type III complexes share the Cas10 subunit but are subclassifed as type IIIA (CSM) and type IIIB (CMR), depending on their specificity for DNA or RNA targets, respectively. The role of CSM in limiting the spread of conjugative plasmids in Staphylococcus epidermidis was first described in 2008. Here, we report a detailed investigation of the composition and structure of the CSM complex from the archaeon Sulfolobus solfataricus, using a combination of electron microscopy, mass spectrometry, and deep sequencing. This reveals a three-dimensional model for the CSM complex that includes a helical component strikingly reminiscent of the backbone structure of the type I (Cascade) family.