EMD-24282

Single-particle
3.68 Å
EMD-24282 Deposition: 22/06/2021
Map released: 04/05/2022
Last modified: 05/06/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-24282

Post-2S intermediate of the Tetrahymena group I intron, symmetry-expanded monomer from a synthetic dimeric construct

EMD-24282

Single-particle
3.68 Å
EMD-24282 Deposition: 22/06/2021
Map released: 04/05/2022
Last modified: 05/06/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Tetrahymena thermophila
Sample: Post-2S intermediate of Tetrahymena group I intron, symmetry-expanded monomer from a synthetic dimeric construct
Fitted models: 7r6m (Avg. Q-score: 0.389)

Deposition Authors: Thelot F, Liu D
Sub-3- angstrom cryo-EM structure of RNA enabled by engineered homomeric self-assembly.
Liu D , Thelot FA, Piccirilli JA , Liao M , Yin P
(2022) Nat Methods , 19 , 576 - 585
PUBMED: 35501384
DOI: doi:10.1038/s41592-022-01455-w
ISSN: 1548-7105
Abstract:
High-resolution structural studies are essential for understanding the folding and function of diverse RNAs. Herein, we present a nanoarchitectural engineering strategy for efficient structural determination of RNA-only structures using single-particle cryogenic electron microscopy (cryo-EM). This strategy-ROCK (RNA oligomerization-enabled cryo-EM via installing kissing loops)-involves installing kissing-loop sequences onto the functionally nonessential stems of RNAs for homomeric self-assembly into closed rings with multiplied molecular weights and mitigated structural flexibility. ROCK enables cryo-EM reconstruction of the Tetrahymena group I intron at 2.98-Å resolution overall (2.85 Å for the core), allowing de novo model building of the complete RNA, including the previously unknown peripheral domains. ROCK is further applied to two smaller RNAs-the Azoarcus group I intron and the FMN riboswitch, revealing the conformational change of the former and the bound ligand in the latter. ROCK holds promise to greatly facilitate the use of cryo-EM in RNA structural studies.