EMD-24346
Negative stain EM map of SARS-CoV-2 Spike in complex with CoVIC-80 IgG
EMD-24346
Single-particle15.0 Å
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Map released: 04/08/2021
Last modified: 10/11/2021
Sample Organism:
Severe acute respiratory syndrome coronavirus 2
Sample: The complex of SARS-CoV-2 Spike and CoVIC-80 IgG
Deposition Authors: Saphire EO, Li H
Sample: The complex of SARS-CoV-2 Spike and CoVIC-80 IgG
Deposition Authors: Saphire EO, Li H
Defining variant-resistant epitopes targeted by SARS-CoV-2 antibodies: A global consortium study.
Hastie KM
,
Li H
,
Bedinger D
,
Schendel SL
,
Dennison SM
,
Li K,
Rayaprolu V
,
Yu X
,
Mann C
,
Zandonatti M
,
Diaz Avalos R,
Zyla D
,
Buck T
,
Hui S
,
Shaffer K
,
Hariharan C
,
Yin J
,
Olmedillas E,
Enriquez A
,
Parekh D
,
Abraha M
,
Feeney E
,
Horn GQ
,
Aldon Y,
Ali H
,
Aracic S
,
Cobb RR
,
Federman RS,
Fernandez JM,
Glanville J
,
Green R,
Grigoryan G
,
Lujan Hernandez AG
,
Ho DD
,
Huang KA
,
Ingraham J
,
Jiang W
,
Kellam P
,
Kim C
,
Kim M
,
Kim HM,
Kong C,
Krebs SJ,
Lan F
,
Lang G,
Lee S,
Leung CL
,
Liu J
,
Lu Y
,
MacCamy A,
McGuire AT
,
Palser AL
,
Rabbitts TH
,
Rikhtegaran Tehrani Z
,
Sajadi MM
,
Sanders RW
,
Sato AK
,
Schweizer L,
Seo J
,
Shen B
,
Snitselaar JL
,
Stamatatos L,
Tan Y
,
Tomic MT,
van Gils MJ
,
Youssef S
,
Yu J,
Yuan TZ
,
Zhang Q
,
Peters B
,
Tomaras GD
,
Germann T
,
Saphire EO
(2021) Science , 374 , 472 - 478
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(2021) Science , 374 , 472 - 478
Abstract:
Antibody-based therapeutics and vaccines are essential to combat COVID-19 morbidity and mortality after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Multiple mutations in SARS-CoV-2 that could impair antibody defenses propagated in human-to-human transmission and spillover or spillback events between humans and animals. To develop prevention and therapeutic strategies, we formed an international consortium to map the epitope landscape on the SARS-CoV-2 spike protein, defining and structurally illustrating seven receptor binding domain (RBD)–directed antibody communities with distinct footprints and competition profiles. Pseudovirion-based neutralization assays reveal spike mutations, individually and clustered together in variants, that affect antibody function among the communities. Key classes of RBD-targeted antibodies maintain neutralization activity against these emerging SARS-CoV-2 variants. These results provide a framework for selecting antibody treatment cocktails and understanding how viral variants might affect antibody therapeutic efficacy.
Antibody-based therapeutics and vaccines are essential to combat COVID-19 morbidity and mortality after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Multiple mutations in SARS-CoV-2 that could impair antibody defenses propagated in human-to-human transmission and spillover or spillback events between humans and animals. To develop prevention and therapeutic strategies, we formed an international consortium to map the epitope landscape on the SARS-CoV-2 spike protein, defining and structurally illustrating seven receptor binding domain (RBD)–directed antibody communities with distinct footprints and competition profiles. Pseudovirion-based neutralization assays reveal spike mutations, individually and clustered together in variants, that affect antibody function among the communities. Key classes of RBD-targeted antibodies maintain neutralization activity against these emerging SARS-CoV-2 variants. These results provide a framework for selecting antibody treatment cocktails and understanding how viral variants might affect antibody therapeutic efficacy.