EMD-24398

Single-particle
3.1 Å
EMD-24398 Deposition: 05/07/2021
Map released: 23/11/2022
Last modified: 29/03/2023
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-24398

Fkbp39 associated nascent 60S class 4

EMD-24398

Single-particle
3.1 Å
EMD-24398 Deposition: 05/07/2021
Map released: 23/11/2022
Last modified: 29/03/2023
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Schizosaccharomyces pombe
Sample: nascent 60S
Fitted models: 8etc (Avg. Q-score: 0.3)

Deposition Authors: Bilokapic S, Halic M
Chromatin localization of nucleophosmin organizes ribosome biogenesis.
Ugolini I, Bilokapic S, Ferrolino M, Teague J, Yan Y, Zhou X, Deshmukh A, White M, Kriwacki RW , Halic M
(2022) Mol Cell , 82 , 4443 - 4457.e9
PUBMED: 36423630
DOI: doi:10.1016/j.molcel.2022.10.033
ISSN: 1097-2765
ASTM: MOCEFL
Abstract:
Ribosome biogenesis takes place in the nucleolus, a nuclear membrane-less organelle. Although well studied, it remains unknown how nascent ribosomal subunits separate from the central chromatin compartment and move to the outer granular component, where maturation occurs. We find that the Schizosaccharomyces pombe nucleophosmin-like protein Fkbp39 localizes to rDNA sites encoding the 60S subunit rRNA, and this localization contributes to its specific association with nascent 60S subunits. Fkbp39 dissociates from chromatin to bind nascent 60S subunits, causing the latter to partition away from chromatin and from nascent 40S subunits through liquid-liquid phase separation. In vivo, Fkbp39 binding directs the translocation of nascent 60S subunits toward the nucleophosmin-rich granular component. This process increases the efficiency of 60S subunit assembly, facilitating the incorporation of 60S RNA domain III. Thus, chromatin localization determines the specificity of nucleophosmin in sorting nascent ribosomal subunits and coordinates their movement into specialized assembly compartments within the nucleolus.