EMD-2583

Single-particle
21.0 Å
EMD-2583 Deposition: 19/02/2014
Map released: 01/07/2015
Last modified: 01/07/2015
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-2583

S. cerevisiae Pex1/6 wild type complex bound to gammaS ATP

EMD-2583

Single-particle
21.0 Å
EMD-2583 Deposition: 19/02/2014
Map released: 01/07/2015
Last modified: 01/07/2015
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Saccharomyces cerevisiae
Sample: S. cerevisiae Pex1/Pex6 wild type complex

Deposition Authors: Ciniawsky S, Grimm I, Saffian D, Girzalsky W, Erdmann R, Wendler P
Molecular snapshots of the Pex1/6 AAA+ complex in action.
Ciniawsky S, Grimm I, Saffian D, Girzalsky W, Erdmann R, Wendler P
(2015) Nat Commun , 6 , 7331
Abstract:
The peroxisomal proteins Pex1 and Pex6 form a heterohexameric type II AAA+ ATPase complex, which fuels essential protein transport across peroxisomal membranes. Mutations in either ATPase in humans can lead to severe peroxisomal disorders and early death. We present an extensive structural and biochemical analysis of the yeast Pex1/6 complex. The heterohexamer forms a trimer of Pex1/6 dimers with a triangular geometry that is atypical for AAA+ complexes. While the C-terminal nucleotide-binding domains (D2) of Pex6 constitute the main ATPase activity of the complex, both D2 harbour essential substrate-binding motifs. ATP hydrolysis results in a pumping motion of the complex, suggesting that Pex1/6 function involves substrate translocation through its central channel. Mutation of the Walker B motif in one D2 domain leads to ATP hydrolysis in the neighbouring domain, giving structural insights into inter-domain communication of these unique heterohexameric AAA+ assemblies.