EMD-26926

Single-particle
4.06 Å
EMD-26926 Deposition: 09/05/2022
Map released: 06/07/2022
Last modified: 17/08/2022
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-26926

Staphylococcus epidermidis RP62A CRISPR effector complex with non-self target RNA 1

EMD-26926

Single-particle
4.06 Å
EMD-26926 Deposition: 09/05/2022
Map released: 06/07/2022
Last modified: 17/08/2022
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Staphylococcus epidermidis, Staphylococcus epidermidis
Sample: Staphylococcus epidermidis RP62a CRISPR effector complex with non-self target RNA

Deposition Authors: Smith EM , Ferrell SH , Tokars VL , Mondragon A
Structures of an active type III-A CRISPR effector complex.
Smith EM , Ferrell S, Tokars VL , Mondragon A
(2022) Structure , 30 , 1109 - 1128.e6
PUBMED: 35714601
DOI: doi:10.1016/j.str.2022.05.013
ISSN: 0969-2126
ASTM: STRUE6
Abstract:
Clustered regularly interspaced short palindromic repeats (CRISPR) and their CRISPR-associated proteins (Cas) provide many prokaryotes with an adaptive immune system against invading genetic material. Type III CRISPR systems are unique in that they can degrade both RNA and DNA. In response to invading nucleic acids, they produce cyclic oligoadenylates that act as secondary messengers, activating cellular nucleases that aid in the immune response. Here, we present seven single-particle cryo-EM structures of the type III-A Staphylococcus epidermidis CRISPR effector complex. The structures reveal the intact S. epidermidis effector complex in an apo, ATP-bound, cognate target RNA-bound, and non-cognate target RNA-bound states and illustrate how the effector complex binds and presents crRNA. The complexes bound to target RNA capture the type III-A effector complex in a post-RNA cleavage state. The ATP-bound structures give details about how ATP binds to Cas10 to facilitate cyclic oligoadenylate production.