EMD-29258

Single-particle
2.47 Å
EMD-29258 Deposition: 21/12/2022
Map released: 12/07/2023
Last modified: 19/07/2023
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-29258

Human nucleolar pre-60S ribosomal subunit (State D1) - Composite map

EMD-29258

Single-particle
2.47 Å
EMD-29258 Deposition: 21/12/2022
Map released: 12/07/2023
Last modified: 19/07/2023
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: human
Sample: Human nucleolar pre-60S ribosomal subunit (State D1)
Fitted models: 8fkv (Avg. Q-score: 0.549)
Raw data: EMPIAR-11472

Deposition Authors: Vanden Broeck A , Klinge S
Principles of human pre-60 S biogenesis.
Vanden Broeck A , Klinge S
(2023) Science , 381 , eadh3892 - eadh3892
PUBMED: 37410842
DOI: doi:10.1126/science.adh3892
ISSN: 1095-9203
ASTM: SCIEAS
Abstract:
During the early stages of human large ribosomal subunit (60S) biogenesis, an ensemble of assembly factors establishes and fine-tunes the essential RNA functional centers of pre-60S particles by an unknown mechanism. Here, we report a series of cryo-electron microscopy structures of human nucleolar and nuclear pre-60S assembly intermediates at resolutions of 2.5 to 3.2 angstroms. These structures show how protein interaction hubs tether assembly factor complexes to nucleolar particles and how guanosine triphosphatases and adenosine triphosphatase couple irreversible nucleotide hydrolysis steps to the installation of functional centers. Nuclear stages highlight how a conserved RNA-processing complex, the rixosome, couples large-scale RNA conformational changes with pre-ribosomal RNA processing by the RNA degradation machinery. Our ensemble of human pre-60S particles provides a rich foundation with which to elucidate the molecular principles of ribosome formation.