EMD-30336

Single-particle
3.2 Å
EMD-30336 Deposition: 12/06/2020
Map released: 28/04/2021
Last modified: 29/05/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-30336

Cryo-EM structure of the flagellar distal rod with partial hook from Salmonella

EMD-30336

Single-particle
3.2 Å
EMD-30336 Deposition: 12/06/2020
Map released: 28/04/2021
Last modified: 29/05/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Sample: Flagellar motor-hook complex
Fitted models: 7cbm (Avg. Q-score: 0.425)

Deposition Authors: Tan JX, Chang SH
Structural basis of assembly and torque transmission of the bacterial flagellar motor.
Tan J, Zhang X, Wang X, Xu C, Chang S, Wu H, Wang T, Liang H, Gao H , Zhou Y, Zhu Y
(2021) Cell , 184 , 2665 - 2679.e19
PUBMED: 33882274
DOI: doi:10.1016/j.cell.2021.03.057
ISSN: 1097-4172
Abstract:
The bacterial flagellar motor is a supramolecular protein machine that drives rotation of the flagellum for motility, which is essential for bacterial survival in different environments and a key determinant of pathogenicity. The detailed structure of the flagellar motor remains unknown. Here we present an atomic-resolution cryoelectron microscopy (cryo-EM) structure of the bacterial flagellar motor complexed with the hook, consisting of 175 subunits with a molecular mass of approximately 6.3 MDa. The structure reveals that 10 peptides protruding from the MS ring with the FlgB and FliE subunits mediate torque transmission from the MS ring to the rod and overcome the symmetry mismatch between the rotational and helical structures in the motor. The LP ring contacts the distal rod and applies electrostatic forces to support its rotation and torque transmission to the hook. This work provides detailed molecular insights into the structure, assembly, and torque transmission mechanisms of the flagellar motor.