EMD-31470
Cryo-EM structure of SARS-CoV-2 spike in complex with a neutralizing antibody chAb-25 (Focused refinement of S-RBD and chAb-25 region)
EMD-31470
Single-particle3.6 Å
Deposition: 24/06/2021Map released: 04/08/2021
Last modified: 16/10/2024
Sample Organism:
Homo sapiens,
Severe acute respiratory syndrome coronavirus 2
Sample: Cryo-EM structure of SARS-CoV-2 spike in complex with a neutralizing antibody chAb-25 (Focused refinement of S-RBD and chAb-25 region)
Fitted models: 7f62 (Avg. Q-score: 0.335)
Deposition Authors: Yang TJ, Yu PY
Sample: Cryo-EM structure of SARS-CoV-2 spike in complex with a neutralizing antibody chAb-25 (Focused refinement of S-RBD and chAb-25 region)
Fitted models: 7f62 (Avg. Q-score: 0.335)
Deposition Authors: Yang TJ, Yu PY
Structure-guided antibody cocktail for prevention and treatment of COVID-19.
Su SC,
Yang TJ,
Yu PY,
Liang KH,
Chen WY,
Yang CW,
Lin HT,
Wang MJ,
Lu RM,
Tso HC,
Chung MJ,
Hsieh TY,
Chang YL,
Lin SC,
Hsu FY,
Ke FY,
Wu YH,
Hwang YC,
Liu IJ,
Liang JJ,
Liao CC 
,
Ko HY,
Sun CP ,
Wu PY,
Jan JT,
Chang YC ,
Lin YL,
Tao MH ,
Hsu SD,
Wu HC
(2021) PLoS Pathog , 17 , e1009704 - e1009704
,
Ko HY,
Sun CP ,
Wu PY,
Jan JT,
Chang YC ,
Lin YL,
Tao MH ,
Hsu SD,
Wu HC
(2021) PLoS Pathog , 17 , e1009704 - e1009704
Abstract:
Development of effective therapeutics for mitigating the COVID-19 pandemic is a pressing global need. Neutralizing antibodies are known to be effective antivirals, as they can be rapidly deployed to prevent disease progression and can accelerate patient recovery without the need for fully developed host immunity. Here, we report the generation and characterization of a series of chimeric antibodies against the receptor-binding domain (RBD) of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein. Some of these antibodies exhibit exceptionally potent neutralization activities in vitro and in vivo, and the most potent of our antibodies target three distinct non-overlapping epitopes within the RBD. Cryo-electron microscopy analyses of two highly potent antibodies in complex with the SARS-CoV-2 spike protein suggested they may be particularly useful when combined in a cocktail therapy. The efficacy of this antibody cocktail was confirmed in SARS-CoV-2-infected mouse and hamster models as prophylactic and post-infection treatments. With the emergence of more contagious variants of SARS-CoV-2, cocktail antibody therapies hold great promise to control disease and prevent drug resistance.
Development of effective therapeutics for mitigating the COVID-19 pandemic is a pressing global need. Neutralizing antibodies are known to be effective antivirals, as they can be rapidly deployed to prevent disease progression and can accelerate patient recovery without the need for fully developed host immunity. Here, we report the generation and characterization of a series of chimeric antibodies against the receptor-binding domain (RBD) of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein. Some of these antibodies exhibit exceptionally potent neutralization activities in vitro and in vivo, and the most potent of our antibodies target three distinct non-overlapping epitopes within the RBD. Cryo-electron microscopy analyses of two highly potent antibodies in complex with the SARS-CoV-2 spike protein suggested they may be particularly useful when combined in a cocktail therapy. The efficacy of this antibody cocktail was confirmed in SARS-CoV-2-infected mouse and hamster models as prophylactic and post-infection treatments. With the emergence of more contagious variants of SARS-CoV-2, cocktail antibody therapies hold great promise to control disease and prevent drug resistance.