EMD-32054

Single-particle
6.9 Å
EMD-32054 Deposition: 14/10/2021
Map released: 19/10/2022
Last modified: 07/12/2022
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-32054

Macroglobulin family protein

EMD-32054

Single-particle
6.9 Å
EMD-32054 Deposition: 14/10/2021
Map released: 19/10/2022
Last modified: 07/12/2022
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Homo sapiens
Sample: macroglobulin family protein

Deposition Authors: Huang X, Wang Y
Cryo-EM structures reveal the dynamic transformation of human alpha-2-macroglobulin working as a protease inhibitor.
Huang X, Wang Y, Yu C, Zhang H , Ru Q , Li X, Song K, Zhou M, Zhu P
(2022) Sci China Life Sci , 65 , 2491 - 2504
PUBMED: 35781771
DOI: doi:10.1007/s11427-022-2139-2
ISSN: 1869-1889
Abstract:
Human alpha-2-macroglobulin is a well-known inhibitor of a broad spectrum of proteases and plays important roles in immunity, inflammation, and infections. Here, we report the cryo-EM structures of human alpha-2-macroglobulin in its native state, induced state transformed by its authentic substrate, human trypsin, and serial intermediate states between the native and fully induced states. These structures exhibit distinct conformations, which reveal the dynamic transformation of alpha-2-macro-globulin that acts as a protease inhibitor. The results shed light on the molecular mechanism of alpha-2-macroglobulin in entrapping substrates.