EMD-33043

Single-particle
7.02 Å
EMD-33043 Deposition: 10/03/2022
Map released: 15/03/2023
Last modified: 20/09/2023
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-33043

CryoEM structure of dsDNA-RuvB-RuvA domain3 complex

EMD-33043

Single-particle
7.02 Å
EMD-33043 Deposition: 10/03/2022
Map released: 15/03/2023
Last modified: 20/09/2023
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: synthetic construct, Pseudomonas aeruginosa PAO1
Sample: RuvB region of the RuvA-RuvB-Holliday junction complex
Fitted models: 7x7p (Avg. Q-score: 0.186)

Deposition Authors: Lin Z , Qu Q , Zhang X , Zhou Z
Cryo-EM structure of the RuvAB-Holliday junction intermediate complex from Pseudomonas aeruginosa.
Zhang X , Zhou Z , Dai L, Chao Y, Liu Z , Huang M, Qu Q , Lin Z
(2023) Front Plant Sci , 14 , 1139106 - 1139106
PUBMED: 37025142
DOI: doi:10.3389/fpls.2023.1139106
ISSN: 1664-462X
Abstract:
Holliday junction (HJ) is a four-way structured DNA intermediate in homologous recombination. In bacteria, the HJ-specific binding protein RuvA and the motor protein RuvB together form the RuvAB complex to catalyze HJ branch migration. Pseudomonas aeruginosa (P. aeruginosa, Pa) is a ubiquitous opportunistic bacterial pathogen that can cause serious infection in a variety of host species, including vertebrate animals, insects and plants. Here, we describe the cryo-Electron Microscopy (cryo-EM) structure of the RuvAB-HJ intermediate complex from P. aeruginosa. The structure shows that two RuvA tetramers sandwich HJ at the junction center and disrupt base pairs at the branch points of RuvB-free HJ arms. Eight RuvB subunits are recruited by the RuvA octameric core and form two open-rings to encircle two opposite HJ arms. Each RuvB subunit individually binds a RuvA domain III. The four RuvB subunits within the ring display distinct subdomain conformations, and two of them engage the central DNA duplex at both strands with their C-terminal β-hairpins. Together with the biochemical analyses, our structure implicates a potential mechanism of RuvB motor assembly onto HJ DNA.