EMD-33692

Single-particle
3.3 Å
EMD-33692 Deposition: 26/06/2022
Map released: 07/09/2022
Last modified: 06/11/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-33692

Structure of the auxin exporter PIN1 in Arabidopsis thaliana in the NPA-bound state

EMD-33692

Single-particle
3.3 Å
EMD-33692 Deposition: 26/06/2022
Map released: 07/09/2022
Last modified: 06/11/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Arabidopsis thaliana, Escherichia coli
Sample: AtPIN1 in complex with a nanobody and NPA
Fitted models: 7y9u (Avg. Q-score: 0.492)

Deposition Authors: Sun L , Liu X , Yang Z, Xia J
Structural insights into auxin recognition and efflux by Arabidopsis PIN1.
Yang Z, Xia J, Hong J, Zhang C, Wei H , Ying W, Sun C, Sun L , Mao Y, Gao Y, Tan S , Friml J , Li D , Liu X , Sun L
(2022) Nature , 609 , 611 - 615
PUBMED: 35917925
DOI: doi:10.1038/s41586-022-05143-9
ISSN: 1476-4687
ASTM: NATUAS
Abstract:
Polar auxin transport is unique to plants and coordinates their growth and development1,2. The PIN-FORMED (PIN) auxin transporters exhibit highly asymmetrical localizations at the plasma membrane and drive polar auxin transport3,4; however, their structures and transport mechanisms remain largely unknown. Here, we report three inward-facing conformation structures of Arabidopsis thaliana PIN1: the apo state, bound to the natural auxin indole-3-acetic acid (IAA), and in complex with the polar auxin transport inhibitor N-1-naphthylphthalamic acid (NPA). The transmembrane domain of PIN1 shares a conserved NhaA fold5. In the substrate-bound structure, IAA is coordinated by both hydrophobic stacking and hydrogen bonding. NPA competes with IAA for the same site at the intracellular pocket, but with a much higher affinity. These findings inform our understanding of the substrate recognition and transport mechanisms of PINs and set up a framework for future research on directional auxin transport, one of the most crucial processes underlying plant development.