EMD-3555
Yeast mitochondrial ribosome - small subunit body (fitted to EMD 3551)
EMD-3555
Single-particle3.3 Å
Deposition: 22/12/2016Map released: 22/02/2017
Last modified: 02/08/2017
Sample Organism:
Saccharomyces cerevisiae
Sample: Yeast mitochondrial ribosome - small subunit body
Raw data: EMPIAR-10107
Deposition Authors: Desai N, Brown A, Amunts A, Ramakrishnan V
Sample: Yeast mitochondrial ribosome - small subunit body
Raw data: EMPIAR-10107
Deposition Authors: Desai N, Brown A, Amunts A, Ramakrishnan V
The structure of the yeast mitochondrial ribosome.
Abstract:
Mitochondria have specialized ribosomes (mitoribosomes) dedicated to the expression of the genetic information encoded by their genomes. Here, using electron cryomicroscopy, we have determined the structure of the 75-component yeast mitoribosome to an overall resolution of 3.3 angstroms. The mitoribosomal small subunit has been built de novo and includes 15S ribosomal RNA (rRNA) and 34 proteins, including 14 without homologs in the evolutionarily related bacterial ribosome. Yeast-specific rRNA and protein elements, including the acquisition of a putatively active enzyme, give the mitoribosome a distinct architecture compared to the mammalian mitoribosome. At an expanded messenger RNA channel exit, there is a binding platform for translational activators that regulate translation in yeast but not mammalian mitochondria. The structure provides insights into the evolution and species-specific specialization of mitochondrial translation.
Mitochondria have specialized ribosomes (mitoribosomes) dedicated to the expression of the genetic information encoded by their genomes. Here, using electron cryomicroscopy, we have determined the structure of the 75-component yeast mitoribosome to an overall resolution of 3.3 angstroms. The mitoribosomal small subunit has been built de novo and includes 15S ribosomal RNA (rRNA) and 34 proteins, including 14 without homologs in the evolutionarily related bacterial ribosome. Yeast-specific rRNA and protein elements, including the acquisition of a putatively active enzyme, give the mitoribosome a distinct architecture compared to the mammalian mitoribosome. At an expanded messenger RNA channel exit, there is a binding platform for translational activators that regulate translation in yeast but not mammalian mitochondria. The structure provides insights into the evolution and species-specific specialization of mitochondrial translation.