EMD-37705

Single-particle
3.4 Å
EMD-37705 Deposition: 08/10/2023
Map released: 13/03/2024
Last modified: 03/04/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-37705

Structure of the Arabidopsis E529Q/E1174Q ABCB19 in the ATP bound state

EMD-37705

Single-particle
3.4 Å
EMD-37705 Deposition: 08/10/2023
Map released: 13/03/2024
Last modified: 03/04/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Arabidopsis thaliana
Sample: Purified E529Q/E1174Q mutant ABCB19 protein in complex with ATP
Fitted models: 8wp0 (Avg. Q-score: 0.464)

Deposition Authors: Ying W , Wei H , Liu X , Sun L
Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export.
Ying W , Wang Y , Wei H , Luo Y , Ma Q , Zhu H, Janssens H , Vukasinovic N , Kvasnica M , Winne JM , Gao Y , Tan S , Friml J , Liu X , Russinova E , Sun L
(2024) Science , 383 , eadj4591 - eadj4591
PUBMED: 38513023
DOI: doi:10.1126/science.adj4591
ISSN: 1095-9203
ASTM: SCIEAS
Abstract:
Brassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. Brassinosteroids are synthesized in the cell interior but bind receptors at the cell surface, necessitating a yet to be identified export mechanism. Here, we show that a member of the ATP-binding cassette (ABC) transporter superfamily, ABCB19, functions as a brassinosteroid exporter. We present its structure in both the substrate-unbound and the brassinosteroid-bound states. Bioactive brassinosteroids are potent activators of ABCB19 ATP hydrolysis activity, and transport assays showed that ABCB19 transports brassinosteroids. In Arabidopsis thaliana, ABCB19 and its close homolog, ABCB1, positively regulate brassinosteroid responses. Our results uncover an elusive export mechanism for bioactive brassinosteroids that is tightly coordinated with brassinosteroid signaling.